Strongyloides venezuelensis is an important alternative source of antigen for the serologic diagnosis of human strongyloidiasis. Proteomics techniques applied to the analysis of the protein content of infective third stage larvae (iL3) of S. venezuelensis provide a powerful tool for the discovery of new candidates for immunodiagnosis. This study presents an overview of the protein iL3 S. venezuelensis focusing on the diagnosis of strongyloidiasis. A total of 877 proteins were identified by shotgun proteomics. Many of these proteins are involved in different cellular processes, metabolic as well as structural maintenance. Our results point to a catalog of possible diagnostic targets for human strongyloidiasis and highlight the need for evaluation of uncharacterized proteins, especially the proteins within the CAP domain, transthyretin, and BTPI inhibitor domains, as a repertoire as yet unexplored in the context of strongyloidiasis diagnostic markers. We believe that the protein profile presented in this shotgun analysis extends our understanding of the protein composition within the Strongyloides genus, opening up new perspectives for research on biomarkers that may help with the diagnosis of human strongyloidiasis. Data are available via ProteomeXchange with identifier PXD013703.

委内瑞拉线虫是用于人类线虫病血清学诊断的重要抗原替代来源。蛋白质组学技术用于分析委内瑞拉链球菌感染性第三阶段幼虫（iL3）的蛋白质含量，为发现新的免疫诊断候选者提供了有力的工具。这项研究概述了蛋白质iL3委内瑞拉葡萄球菌。侧重于线虫病的诊断。通过shot弹枪蛋白质组学鉴定出总共877种蛋白质。这些蛋白质中的许多涉及不同的细胞过程，代谢以及结构维持。我们的研究结果指向了人类圆线虫病的可能诊断目标的目录，并强调了需要评估未表征的蛋白质，尤其是CAP域，运甲状腺素蛋白和BTPI抑制剂域中的蛋白质，作为在圆线虫病诊断背景下尚未探索的所有文献标记。我们相信，这种shot弹枪分析中显示的蛋白质谱扩展了我们对强茎线虫中蛋白质组成的理解属，为生物标志物的研究开辟了新的视角，可能有助于诊断人类圆线虫病。数据可通过ProteomeXchange获得，其标识符为PXD013703。