Sensitive cathodic stripping voltammetric methods have been developed for two quinolone antibacterial drugs, pipemidic acid (PIP) and ofloxacin (OFL) using hanging mercury drop electrode as working electrode vs. Ag/AgCl reference electrode. The methods were developed for the determination of drugs individually as well as simultaneously. 0.1 M and 0.01 M hydrochloric acid was used as medium for PIP and OFL, respectively, 0.1 M potassium chloride was used as base electrolyte. Reduction waves were observed for PIP within −700 mV to −800 mV and for OFL within −1100 mV to −1200 mV. Linear calibration ranges for PIP and OFL were observed within 10–100 μg ml⁻¹ with detection limits of 50 ng ml⁻¹ and 1 μg ml⁻¹, respectively. Relative standard deviations (RSD) for the analysis of 10 gµg ml⁻¹ of PIP and OFL (n = 6) were 0.5% and 1.4%, respectively. The presence of glucose, lactose, sorbitol, gum arabic, starch, magnesium stearate, methylparaben and propylparaben did not affect the determinations of both PIP and OFL. The methods were used for the analysis of pharmaceutical preparations and the results indicated relative deviation of 0.5–5.5% from labeled values with RSD within 0.49–2.5%. PIP and OFL could also be determined simultaneously, and were determined from spiked human urine.

中文翻译：

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药物剂量和人尿液中哌啶酸和氧氟沙星的阴极溶出伏安法

敏感阴极溶出伏安方法已经被开发用于使用悬汞电极为工作电极2种喹诺酮类抗菌药，吡哌酸（PIP）和氧氟沙星（OFL）VS。Ag / AgCl参比电极。开发了用于分别或同时测定药物的方法。0.1 M和0.01 M盐酸分别用作PIP和OFL的介质，0.1 M氯化钾用作基础电解质。对于-700 mV至-800 mV的PIP，对于-1100 mV至-1200 mV的OFL，观察到了还原波。在10–100μgml ^-1范围内观察到PIP和OFL的线性校准范围，检出限为50 ng ml ^-1和1μgml ^-1， 分别。用于分析10 gµg ml ^-1的PIP和OFL（n = 6）的相对标准偏差（RSD）分别为0.5％和1.4％。葡萄糖，乳糖，山梨糖醇，阿拉伯树胶，淀粉，硬脂酸镁，对羟基苯甲酸甲酯和对羟基苯甲酸丙酯的存在不会影响PIP和OFL的测定。该方法用于药物制剂的分析，结果表明相对于标记值的相对偏差为0.5–5.5％，RSD在0.49–2.5％之内。PIP和OFL也可以同时测定，并从加标的人尿中测定。