Teprenone (geranylgeranylacetone) is one kind of safe and effective agent in gastrointestinal mucosa, which have been widely used in human and veterinary, but rarely used in aquaculture animals. In this study, Lateolabrax maculatus, an important economic fish species in southern China, was taken as the object of study to investigate the protective effect of teprenone on intestinal stress. The present study was designed to investigate the potential mechanism underlying the protection offered by teprenone to protect the gastrointestinal tract against hypoxia and reoxygenation injury of L. maculatus. (a) For oxidative stress parameters, SOD, CAT, and T-AOC in control group were higher than those in teprenone group. MDA content was significantly higher than that in teprenone group at N and 12h time points in intestine (P < 0.05), and at 12, 24, and 48 h time points in stomach. (b) For immune-associated proteins, LZM activity in the control group was lower than that in the teprenone group, and the difference between the two groups in stomach and intestine was significant at 12.48 h and 6.48 h time points, respectively (P < 0.05). Compared with time point N, the content of HSP70 in the control group increased at 0 h in intestine. At 0–48 h, intestine HSP70 content in the control group showed a gradually decreasing trend, which was higher than that in the teprenone group. (c) For apoptosis-related factors, the activity of Cyt-C, caspase9, and caspase3 increased first and then decreased in both groups. The content of Cyt-C in the control group was significantly higher than that in the teprenone group at N-3.6 h, and 3.48 h time points in stomach and intestine, respectively (P<0.05). The activity of caspase9 and caspase3 was higher than that in the teprenone group at N-48 h. Results indicated that acute hypoxia and reoxygenation cause the expression levels of oxidative stress and apoptosis-related factors in the stomach and intestine increased first and then decreased within 0–48 h. Acute hypoxia and reoxygenation also that causes the level of nonspecific immunity decreased first and then increased. A total of 400-mg/kg treatment of teprenone can protect stomach and intestinal tissues to a certain extent. It can effectively protect oxidative stress and apoptosis within 0–48 h after acute hypoxia and reoxygenation and enhance non-specific immunity.

甲泼尼龙（香叶基香叶基丙酮）是胃肠道粘膜中一种安全有效的药物，已广泛用于人类和兽医，但很少用于水产养殖动物。本研究以华南地区重要的经济鱼类蓝斑豚为研究对象，以研究替普利酮对肠道应激的保护作用。本研究旨在研究潜在的机制，由替普利酮提供保护，以保护胃肠道免受低氧和黄斑狼疮的氧合损伤。。（a）在氧化应激参数方面，对照组中的SOD，CAT和T-AOC高于替普利酮组。在肠的N和12h时间点以及胃的12、24和48h时间点，MDA含量均显着高于替普利酮组（P <0.05）。（b）对于免疫相关蛋白，对照组的LZM活性低于替普利酮组，两组的胃和肠差异分别在12.48h和6.48h的时间点显着（P<0.05）。与时间点N相比，对照组的HSP70含量在肠内0 h时增加。在0-48 h，对照组小肠HSP70含量呈逐渐下降趋势，高于替普利酮组。（c）对于凋亡相关因子，两组中Cyt-C，caspase9和caspase3的活性先升高后降低。在胃和肠的N-3.6 h和3.48 h时间点，对照组的Cyt-C含量显着高于替普利酮组（P<0.05）。在N-48 h，caspase9和caspase3的活性高于替普利酮组。结果表明，急性缺氧和复氧导致胃和肠中氧化应激和细胞凋亡相关因子的表达水平先升高后在0–48 h内降低。急性缺氧和复氧也会导致非特异性免疫水平先降低然后升高。总剂量为400 mg / kg的替普利酮可以在一定程度上保护胃和肠组织。它可以有效保护急性缺氧和复氧后0-48小时内的氧化应激和细胞凋亡，并增强非特异性免疫。