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Genetic Variation in Genes Involved in Ethanol Production Among Saccharomyces cerevisiae Strains
Sugar Tech ( IF 1.9 ) Pub Date : 2019-09-27 , DOI: 10.1007/s12355-019-00771-4
Sunisa Chatsurachai , Nantida Watanarojanaporn , Saranya Phaengthai , Morakot Sakulsombat , Klanarong Sriroth

Saccharomyces cerevisiae has been known for its applications in a wide range of industries such as food and beverages, pharmaceuticals, bioethanol and feed industries. Mitr Phol Innovation and Research Center has successfully isolated and developed a few Saccharomyces cerevisiae strains for their ethanol plants located in four different regions of Thailand. Industrial ethanol production in Thailand is typically conducted without a strictly aseptic bioreactor using molasses as the feedstock. In order to increase ethanol production and its efficiency, the yeast strains require the ability to tolerate ethanol concentrations higher than 10% (ethanol v/v) and stresses from molasses. Since the conventional method to isolate yeast strains from natural resources is laborious, random and costly, it is necessary to use an alternative method using bioinformatics and systems biology for better understanding of the genetic–phenotypic relationship that helps to discover target genes for yeast improvement. It was found that our isolated strains (MP11, MP15) show high ethanol production compared with the commercial strain Angel Super Alcohol (Angel Yeast, Co. Ltd.). Therefore, we selected 10 important genes (ADH1, ADH3, ADH4, ADH5, HXT1, HXT2, HXT3, HXT4, TPI1 and SUC2) based on previous publications to clone and sequence candidate genes of Mitr Phol’s yeast strains. After that, we applied comparative sequence analysis among yeast strains to identify DNA variation in genes that are relevant to ethanol production. From the results, the most variation of DNA sequence was found in a primary enzyme for ethanol production (ADH1) of about 12%. In addition, the variation of ADH1 was also found at substrate binding sites among our isolated and commercial strains. This result implies that genetic variation among yeast strains has an effect on ethanol production especially on the key enzyme in the ethanol pathway. In order to implement yeast strains with the specific trait to improve ethanol production, genetic information of each yeast strain is required to further apply this strategy for stress tolerance traits.

中文翻译:

酿酒酵母菌株中涉及乙醇生产的基因的遗传变异

酿酒酵母因其在食品和饮料,制药,生物乙醇和饲料工业等广泛行业中的应用而闻名。Mitr Phol创新研究中心已成功分离并开发了几种酿酒酵母位于泰国四个不同地区的乙醇工厂的菌株。在泰国,工业乙醇生产通常无需使用糖蜜作为原料的严格无菌生物反应器进行。为了增加乙醇的产量及其效率,酵母菌株需要能够耐受高于10%(乙醇v / v)的乙醇浓度和糖蜜的压力。由于从自然资源中分离酵母菌株的常规方法费力,随机且成本高昂,因此有必要使用一种利用生物信息学和系统生物学的替代方法来更好地了解有助于发现酵母改良目标基因的遗传-表型关系。发现我们分离出的菌株(MP11,与商业菌株Angel Super Alcohol(Angel Yeast,Co.Ltd。)相比,MP15)显示出较高的乙醇产量。因此,我们选择了10个重要基因(ADH1ADH3ADH4ADH5HXT1HXT2HXT3HXT4TPI1SUC2基于以前的出版物,以MITR Phol的酵母菌株的克隆和序列候选基因)。此后,我们在酵母菌株中应用了比较序列分析,以鉴定与乙醇生产相关的基因中的DNA变异。根据结果​​,在乙醇生产的主要酶(ADH1)中发现最大的DNA序列变异约为12%。另外,ADH1的变异在我们分离的和商业菌株中,在底物结合位点也发现了这种蛋白。该结果表明,酵母菌株之间的遗传变异对乙醇产生特别是对乙醇途径中的关键酶具有影响。为了实施具有特定性状的酵母菌株以改善乙醇的产生,需要每个酵母菌株的遗传信息以进一步将该策略用于胁迫耐受性状。
更新日期:2019-09-27
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