Calcium is an element that performs many important functions in the human body. A study was conducted on the use of a pulsed electric field (PEF) to enrich cells of Lactobacillus rhamnosus B 442 in calcium ions. The highest concentration of calcium ions in bacterial cells (7.30 mg/g d.m.) was obtained at ion concentration of 200 µg/ml of medium and with the use of the following PEF parameters: field strength 3.0 kV/cm, exposure time 10 min, pulse width 75 ms and 20 h of culturing after which bacteria were treated with the field. Cell biomass varied in the range from 0.09 g/g d.m. to 0.252 g/g d.m., and the total number of bacteria ranged from 10¹⁰ CFU/ml to 10¹² CFU/ml. Microscope photographs prove that calcium ions were situated within the cells of the bacteria, and electroporation contributed to an increase in the effectiveness of the ion bioaccumulation process. Samples containing calcium and subjected to electroporation displayed intensive fluorescence. The significance of this research was the possibility of using probiotic bacteria enriched with calcium ions for the production of functional food in subsequent studies.

中文翻译：

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脉冲电场对鼠李糖乳杆菌 B 442 中钙积累的影响。

钙是一种在人体中发挥许多重要功能的元素。进行了一项关于使用脉冲电场 (PEF) 富集钙离子的鼠李糖乳杆菌B 442 细胞的研究。细菌细胞中钙离子的最高浓度 (7.30 mg/g dm) 是在培养基的离子浓度为 200 µg/ml 并使用以下 PEF 参数时获得的：场强 3.0 kV/cm，曝光时间 10 分钟，脉冲宽度 75 毫秒和 20 小时的培养，然后用场处理细菌。细胞生物量在 0.09 g/g dm 到 0.252 g/g dm 之间变化，细菌总数在 10 ¹⁰ CFU/ml 到 10 ¹²菌落形成单位/毫升。显微镜照片证明钙离子位于细菌细胞内，电穿孔有助于提高离子生物积累过程的有效性。含有钙并经过电穿孔的样品显示出强烈的荧光。这项研究的意义在于，在后续研究中可能使用富含钙离子的益生菌来生产功能性食品。