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Integrated selection and identification of bacteria from polluted sites for biodegradation of lipids.
International Microbiology ( IF 3.1 ) Pub Date : 2019-12-11 , DOI: 10.1007/s10123-019-00109-w Pedro D Teixeira 1, 2 , Vitor S Silva 2 , Rogério Tenreiro 1
International Microbiology ( IF 3.1 ) Pub Date : 2019-12-11 , DOI: 10.1007/s10123-019-00109-w Pedro D Teixeira 1, 2 , Vitor S Silva 2 , Rogério Tenreiro 1
Affiliation
Wastewater treatment plants face major social concern towards removal of problematic pollutants such as fat oils and grease (FOG). In this context, the main objective of the present work was to select natural bacterial isolates from different polluted sites and evaluate them comparatively to isolates from commercial products, for improved bioremediation strategies and bioaugmentation. In total, 196 isolates were analysed for genomic diversity by two PCR-fingerprinting methods and screened for biodegradation potential with pollutants as sole carbon source. The net area under curve (NAUC) was used for preliminary evaluation of growth ability in M9 medium supplemented with oleic acid and triolein. A principal component analysis of all NAUC data showed that natural isolates presented higher overall biodegradation ability and enabled the selection of 11 natural isolates for lipid degradation assays. Selected isolates were identified by 16S rRNA gene sequencing as members of genera with previously described degradative strains, namely, Acinetobacter (1), Aeromonas (2), Bacillus (1), Pseudomonas (1) and Staphylococcus (6). Best biodegradation results in 7-days assay of FOG content removal were 37.9% for oleic acid and 19.1% for triolein by an Aeromonas sp. isolate and a Staphylococcus cohnii isolate, respectively. A respirometry approach confirmed their higher oxygen uptake rates, although longer adaptation phases where required by the Aeromonas sp. isolate. Consequently, these isolates showed great potential for future bioaugmentation products, to promote FOG degradation, for both in situ and ex situ approaches.
中文翻译:
综合选择和鉴定来自受污染地点的细菌,以进行脂质的生物降解。
废水处理厂在去除有问题的污染物(例如脂肪油和油脂(FOG))方面面临着重大的社会关注。在这种情况下,当前工作的主要目的是从不同污染场所中选择天然细菌分离物,并对它们与商品中的分离物进行比较评估,以改善生物修复策略和生物强化。共有196种分离物通过两种PCR指纹分析方法进行了基因组多样性分析,并筛选了以污染物为唯一碳源的生物降解潜力。曲线下的净面积(NAUC)用于初步评估在补充有油酸和三油精的M9培养基中的生长能力。对所有NAUC数据的主成分分析表明,天然分离物具有更高的整体生物降解能力,能够选择11种天然分离物用于脂质降解测定。通过16S rRNA基因测序,将选定的分离物鉴定为先前描述的降解菌株的属,即不动杆菌(1),气单胞菌(2),芽孢杆菌(1),假单胞菌(1)和葡萄球菌(6)。在7天的FOG含量去除试验中,最佳生物降解结果是:Aeromonas sp对油酸为37.9%,对三油精为19.1%。分离株和金黄色葡萄球菌分离株。呼吸测定法证实了它们较高的氧气吸收率,尽管在气单胞菌属物种需要的适应期较长。隔离株。因此,这些分离物在原位和异位方法中都显示出巨大的潜力,可用于未来的生物增强产品,促进FOG降解。
更新日期:2019-12-11
中文翻译:
综合选择和鉴定来自受污染地点的细菌,以进行脂质的生物降解。
废水处理厂在去除有问题的污染物(例如脂肪油和油脂(FOG))方面面临着重大的社会关注。在这种情况下,当前工作的主要目的是从不同污染场所中选择天然细菌分离物,并对它们与商品中的分离物进行比较评估,以改善生物修复策略和生物强化。共有196种分离物通过两种PCR指纹分析方法进行了基因组多样性分析,并筛选了以污染物为唯一碳源的生物降解潜力。曲线下的净面积(NAUC)用于初步评估在补充有油酸和三油精的M9培养基中的生长能力。对所有NAUC数据的主成分分析表明,天然分离物具有更高的整体生物降解能力,能够选择11种天然分离物用于脂质降解测定。通过16S rRNA基因测序,将选定的分离物鉴定为先前描述的降解菌株的属,即不动杆菌(1),气单胞菌(2),芽孢杆菌(1),假单胞菌(1)和葡萄球菌(6)。在7天的FOG含量去除试验中,最佳生物降解结果是:Aeromonas sp对油酸为37.9%,对三油精为19.1%。分离株和金黄色葡萄球菌分离株。呼吸测定法证实了它们较高的氧气吸收率,尽管在气单胞菌属物种需要的适应期较长。隔离株。因此,这些分离物在原位和异位方法中都显示出巨大的潜力,可用于未来的生物增强产品,促进FOG降解。