Marine snails are abundant sources of biologically important conopeptides with their potential applications in drug development. The present study aimed to identify the potential conopeptides from the venom duct of Conus inscriptus. After extraction conopeptides were characterized by liquid chromatography–mass spectrometric analysis showing totally 29 protein sequences with disulfide linkages of different molecular mass distributed in the range of 387–1536 m/z and the peptides showing mostly to T-superfamily of conotoxins with 78 kDa heat shock proteins. The venom peptides showed six different molecular weight bands (37, 51, 60, 70, 80 and 90 kDa) above 30 kDa after in-gel enzymatic digestion. Furthermore, the conopeptides exhibit potential cytotoxic activity against HeLa-HPV 16 associated, Vero (normal) cell lines and brine shrimp. Fourier transform infra-red spectroscopy analysis confirms the structural and functional groups. The observed results suggests the venom peptides from C. inscriptus as a potential anticancer agent.

中文翻译：

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圆锥体刀豆毒素蛋白的分离，鉴定及其对宫颈癌（HeLa-HPV 16相关细胞）的潜在抗癌活性

海洋蜗牛是生物学上重要的coneptepteptes的丰富来源，其在药物开发中的潜在应用。本研究的目的是从圆锥体蛇毒的毒液管中鉴定出潜在的conopepteptides。。提取后，通过液相色谱-质谱分析对共肽进行了表征，共显示29种具有不同分子量的二硫键的蛋白质序列，分布在387-1536 m / z的范围内，并且这些肽大部分显示为伴有毒素的T超家族，热量为78 kDa休克蛋白。凝胶内酶消化后，蛇毒肽在30 kDa以上显示六个不同的分子量带（37、51、60、70、80和90 kDa）。此外，conopepteptide对HeLa-HPV 16相关的Vero（正常）细胞系和盐水虾显示出潜在的细胞毒活性。傅立叶变换红外光谱分析证实了结构和官能团。观察到的结果表明，来自C. inscriptus的毒液肽是潜在的抗癌药。