The vaginal microbiota may be important for pregnancy prognosis because vaginal dysbiosis during pregnancy appears to be related to preterm birth (PTB) or pregnancy loss. Previous reports have indicated that a Lactobacillus-poor microbial flora in the vagina and intrauterine infection by diverse anaerobes ascending from the vagina are associated with undesirable delivery outcomes. However, no research has involved the use of pyrosequencing analysis to examine vaginal microbiota profiles or their potential associations with high-risk pregnancy in Korean women. Vaginal swabs were collected from 500 Korean women for the identification of community state types (CSTs). Of these, 137 samples were further analyzed using a Roche/454 GS Junior pyrosequencer. Three distinct CSTs were identified based on the dominant vaginal microbes: CST I (Lactobacillus crispatus dominated), CST III (Lactobacillus iners dominated), and CST IV (with diverse species of anaerobes). Twelve of the 67 pregnant women had undesirable pregnancy outcomes (four miscarriages and eight PTBs). The dominant microbe in the vaginal microbiota of women who gave birth at full-term was L. crispatus. In contrast, L. iners was the dominant vaginal microbe in women who miscarried. Most (n = 6/8) vaginal microbiota profiles of women who experienced PTB could be classified as CST IV, with diverse bacteria, including anaerobic vaginal species. The present study provides valuable information regarding the characteristics of the vaginal microbiota of Korean women related to high-risk pregnancy. Investigation of the vaginal microbiotic structure in pregnant Korean women is necessary to enable better prediction of adverse pregnancy outcomes.

中文翻译：

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韩国本土妇女的阴道微生物群概况和高危妊娠协会。

阴道微生物群可能对妊娠预后很重要，因为妊娠期间的阴道菌群失调似乎与早产 (PTB) 或流产有关。以前的报告表明，乳酸杆菌- 阴道微生物群落和从阴道上升的各种厌氧菌引起的宫内感染与不良分娩结果有关。然而，还没有研究涉及使用焦磷酸测序分析来检查韩国女性的阴道微生物群概况或其与高危妊娠的潜在关联。收集了 500 名韩国女性的阴道拭子，用于鉴定社区状态类型 (CST)。其中，137 个样品使用 Roche/454 GS Junior 焦磷酸测序仪进行了进一步分析。根据主要的阴道微生物确定了三种不同的 CST：CST I（以卷曲乳杆菌为主）、CST III（惰性乳杆菌占主导地位）和 CST IV（具有多种厌氧菌）。67 名孕妇中有 12 名有不良妊娠结局（4 名流产和 8 名 PTB）。足月分娩妇女阴道微生物群中的优势微生物是L. crispatus。相比之下，惰性乳杆菌是流产女性的主要阴道微生物。大多数 ( n= 6/8) 经历过 PTB 的女性的阴道微生物群概况可归类为 CST IV，具有多种细菌，包括厌氧阴道菌种。本研究提供了有关韩国高危妊娠女性阴道微生物群特征的宝贵信息。有必要调查韩国孕妇的阴道微生物结构，以便更好地预测不良妊娠结局。