Skin injury leading to chronic wounds is of high interest due to the increasing number of patients suffering from this symptom. Proliferation, migration, and angiogenesis are key factors in the wound healing processes. For that reason, controlled promotion of these processes is required. In this work, we present the portable helium-dielectric barrier discharge (He-DBD)-based reaction-discharge system of controlled gas temperature for biological activities. To make this He-DBD-based reaction-discharge system safe for biological purposes, a multivariate optimization of the operating parameters was performed. To evaluate the effect of the He-DBD operating parameters on the rotational gas temperature T rot (OH), a design of experiment followed by a Response Surface Methodology was applied. Based on the suggested statistical model, the optimal operating conditions under which the T rot (OH) is less than 37 °C (310 K) were estimated. Then, the resulted model was validated in order to confirm its accuracy. After estimation the optical operating conditions of He-DBD operation, the spectroscopic characteristic of the He-DBD-based reaction-discharge system in relevance to the several optical temperatures in addition to electron number density has been carried out. Additionally, the qualitative and quantitative analyses of the reactive oxygen species and reactive nitrogen species were performed in order to investigate of reactions and processes running in the He-DBD-gaseous phase and in the He-DBD-treated liquid. Next, the developed portable He-DBD-based reaction-discharge system, working under the optimal operating conditions, was used to stimulate the wound healing process. It was found that a 30 s He-DBD treatment significantly increased the proliferation, migration, and angiogenesis of keratinocytes (HaCaT) and fibroblasts (MSU-1.1) cell lines, as well as human skin microvascular endothelial cells (HSkMEC.2). Hence, the application of the cold atmospheric pressure plasma generated in this He-DBD-based reaction-discharge system might be an alternative therapy for patient suffering from chronic wounds.

中文翻译：

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通过便携式介电势垒放电反应放电系统激活正常人体皮肤细胞，并确定气体温度

由于患有这种症状的患者数量不断增加，导致慢性伤口的皮肤损伤备受关注。增殖、迁移和血管生成是伤口愈合过程中的关键因素。因此，需要有控制地促进这些过程。在这项工作中，我们提出了基于便携式氦-电介质阻挡放电（He-DBD）的生物活动可控气体温度反应放电系统。为了使这种基于 He-DBD 的反应放电系统安全用于生物学目的，对操作参数进行了多变量优化。为了评估 He-DBD 操作参数对旋转气体温度 T rot (OH) 的影响，应用了实验设计，然后是响应面方法。根据建议的统计模型，估计了 T rot (OH) 小于 37 °C (310 K) 的最佳操作条件。然后，对得到的模型进行验证，以确认其准确性。在估计了 He-DBD 操作的光学操作条件后，除了电子数密度之外，还进行了基于 He-DBD 的反应放电系统的光谱特性与几个光学温度的相关性。此外，还对活性氧和活性氮进行了定性和定量分析，以研究在 He-DBD 气相和 He-DBD 处理的液体中运行的反应和过程。接下来，开发的基于 He-DBD 的便携式反应放电系统在最佳操作条件下工作，用于刺激伤口愈合过程。结果发现，30 秒 He-DBD 治疗显着增加了角质形成细胞 (HaCaT) 和成纤维细胞 (MSU-1.1) 以及人类皮肤微血管内皮细胞 (HSkMEC.2) 的增殖、迁移和血管生成。因此，在这种基于 He-DBD 的反应放电系统中产生的冷大气压等离子体的应用可能是慢性伤口患者的替代疗法。