Esophageal squamous cell carcinoma (ESCC) is a disease with poor prognosis which urgently is in need of effective prognostic marker. To discover novel prognostic protein marker for ESCC, we applied a high-throughput monoclonal antibody microarray to compare tumor and adjacent non-tumor tissues from ESCC patients. Antibody #ESmAb270 was consistent higher expressed in tumors and it was identified via mass spectrometry to be stromal interaction molecule 1 (STIM1). STIM1 H scores in tumor tissues were significantly up-regulated in esophageal tumor tissues compared to non-tumor tissues in 105 ESCC patients. We also observed that high STIM1 expression was correlated with advanced tumor grade and poor prognosis of ESCC. In addition, attenuation of STIM1 by siRNA or chemical inhibitors significantly inhibited cell viability and migration of ESCC cells. Evidence from high-throughput monoclonal antibody microarray, IHC microarray with associated survival data and functional analysis show that STIM1 is an unfavorable prognostic biomarker in ESCC.

食管鳞状细胞癌（ESCC）是一种预后较差的疾病，迫切需要有效的预后指标。为了发现ESCC的新型预后蛋白标志物，我们应用了高通量单克隆抗体微阵列来比较ESCC患者的肿瘤和邻近的非肿瘤组织。抗体＃ESmAb270始终在肿瘤中高表达，并通过质谱鉴定为基质相互作用分子1（STIM1）。与105例ESCC患者中的非肿瘤组织相比，食管肿瘤组织中肿瘤组织中的STIM1 H分数显着上调。我们还观察到高STIM1表达与晚期肿瘤分级和ESCC预后不良相关。此外，通过siRNA或化学抑制剂对STIM1的衰减显着抑制了细胞活力和ESCC细胞的迁移。