Actinopyga mauritiana, Bohadschia marmorata, Holothuria leucospilota, H. edulis, H. atra and H. polii are the abundant sea-cucumbers inhabiting either Red or Mediterranean Sea shore in Egypt. The aim of this study was to elucidate the metabolic content in the tegument of the selected sea-cucumbers using ultra-performance liquid chromatography coupled to high-resolution mass spectrometry (UPLC/HRMS). The identified metabolites included sulfated saponins such as holothurin B1 and 24-dehydroechinoside A and non-sulfated saponins such as bivittoside C and D. Our results showed that B. marmorata is substantially different from all the other species and occupies alone one taxon. Consequently, it can be suggested to reclassify the family Holothuriidae into three subfamilies. The first suggested comprising the genera, Holothuria and Actinopyga, the second includes only genus Bohadschia, whereas the third includes only genus Pearsonothuria. Lipidomic analysis of sea-cucumbers showed high variation in saturated and unsaturated fatty acids (USFAs) between the collected species. H. edulis and B. marmorata showed the highest amount of all identified SFAs. Among the USFAs, palmitoleic, docosatrienoic and linoleic acid were detected in varied amount in most species.

中文翻译：

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使用超高效液相色谱（UPLC）结合高分辨率质谱（HRMS）对最丰富的埃及海参进行化学分类学研究

辐圣莫尔itiana，图纹白尼参，玉足海参，^ h。牛肝菌，H. ATRA和H. polii是丰富的海洋-海参栖息红色或在埃及地中海岸。本研究的目的是阐明在所选择的海的皮层代谢内容-使用耦合到高分辨率质谱（UPLC / HRMS）超高效液相色谱黄瓜。所识别的代谢物包括的硫酸化皂苷如海参素B1和24 dehydroechinoside A和非硫酸化的皂甙如bivittoside C和D.我们的结果表明，B. marmorata与其他物种完全不同，仅占用一个分类单元。因此，建议将虎耳科分为三个亚科。第一个建议包括Holothuria和Actinopyga属，第二个建议仅包括Bohadschia属，而第三个建议仅包括Pearsonothuria属。海参的脂质学分析显示，所收集物种之间的饱和脂肪酸和不饱和脂肪酸（USFA）差异很大。H. edulis和B. marmorata在所有已鉴定的SFA中含量最高。在USFA中，在大多数物种中检测到棕榈油酸，二十二碳三烯酸和亚油酸的量各不相同。