The chronological development of procedures for determining the cholesterol concentration of in plasma, serum, and whole blood is presented in the review. It is stated that, since the correlation between the risk of development of cardiovascular diseases and the concentrations of total cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol in human blood was established by numerous medical studies, procedures for the measurement of these parameters have been developed most actively. A brief overview of these procedures and the results of their comparative testing in patient medical examinations are given. Classifications are proposed for procedures for determining total cholesterol, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol in the blood. The mechanism of action of the chemical reactions taking place in these procedures, advantages and disadvantages, and priorities for their application are considered. Promising directions for the development and improvement of procedures to ensure more accurate measurements of the blood cholesterol concentration, are mentioned, and alternative means of determining the risk of cardiovascular disease are discussed.

中文翻译：

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血液中胆固醇的测定。第2部分

本综述介绍了按时间顺序开发的测定血浆，血清和全血中胆固醇浓度的程序。据指出，由于心血管疾病发展的风险与人血中总胆固醇，低密度脂蛋白胆固醇和高密度脂蛋白胆固醇的浓度之间的相关性是通过众多医学研究建立的，因此测量这些参数的开发最为活跃。简要概述了这些程序及其在患者体检中进行的比较测试的结果。提出了用于确定血液中总胆固醇，低密度脂蛋白胆固醇和高密度脂蛋白胆固醇的程序的分类。考虑了在这些程序中发生的化学反应的作用机理，优缺点和应用优先级。提到了开发和改进程序的有希望的方向，以确保更准确地测量血胆固醇浓度，并讨论了确定心血管疾病风险的替代方法。