The present work aimed to estimate the possible anti-fatigue effect and potential mechanism of Isochrysis galbana (IG) in mice. The anti-fatigue activity of IG (100, 200, and 400 mg/kg) was elucidated by a weight-loaded forced swimming test, and the potential mechanism was explored by determination of fatigue-related biochemical parameters. The results showed that pretreatment with IG significantly extended the exhaustive swimming time and increased the levels of liver glycogen, muscle glycogen and blood glucose in a dose-dependent manner. Besides, the increased levels of alanine aminotransferase, aspartate aminotransferase, blood lactic acid, lactic dehydrogenase, creatine kinase, and blood urea nitrogen by exhausted swimming, were dramatically attenuated by pretreatment with IG. Furthermore, supplementation with IG significantly enhanced the glutathione peroxidase and superoxide dismutase levels, while attenuated the level of malonaldehyde. Taken together, IG possessed appreciable efficacy to alleviate fatigue, and the mechanism might be associated with favorably modulating the process of energy consumption, metabolism, and attenuating oxidative stress injury.

中文翻译：

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评价海洋微藻等鞭金藻在小鼠中的抗疲劳和抗氧化活性。

目前的工作旨在估计可能的抗疲劳作用和等轴线虫（IG）在小鼠中的潜在机制。通过负重游泳试验阐明了IG（100、200和400 mg / kg）的抗疲劳活性，并通过确定疲劳相关的生化参数来探索其潜在的机制。结果表明，IG预处理显着延长了力竭的游泳时间，并以剂量​​依赖性方式增加了肝糖原，肌肉糖原和血糖的水平。此外，用IG预处理可显着减轻因疲惫的游泳而引起的丙氨酸转氨酶，天冬氨酸转氨酶，血乳酸，乳酸脱氢酶，肌酸激酶和血尿素氮水平的升高。此外，IG的补充显着提高了谷胱甘肽过氧化物酶和超氧化物歧化酶的水平，同时减弱了丙二醛的水平。综上所述，IG具有缓解疲劳的显着功效，其机理可能与调节能量消耗，新陈代谢和减轻氧化应激损伤的过程有关。