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Growth and nitrogen removal characteristics of Halomonas sp. B01 under high salinity
Annals of Microbiology ( IF 3 ) Pub Date : 2019-11-18 , DOI: 10.1007/s13213-019-01526-y Te Wang , Zhengzhong Jiang , Wenbo Dong , Xiaoya Liang , Linghua Zhang , Yimin Zhu
Annals of Microbiology ( IF 3 ) Pub Date : 2019-11-18 , DOI: 10.1007/s13213-019-01526-y Te Wang , Zhengzhong Jiang , Wenbo Dong , Xiaoya Liang , Linghua Zhang , Yimin Zhu
At present, the nitrogen (N) removal efficiency of the microbial treatment in the high-salinity nitrogenous wastewaters is relatively low. Study on the N removal behavior and properties of moderately halophilic bacteria Halomonas under high salinity is of great significance for the microbial treatment of high-salinity nitrogenous wastewater. The response mechanism of Halomonas sp. B01 to high osmotic pressure stress was investigated by measuring the compatible solute ectoine concentration and superoxide dismutase (SOD) activity. The salt tolerance during growth and N removal of the strain was evaluated by measuring the activities of growth-related and N removal–related enzymes and the mRNA expression abundance of ammonia monooxygenase-encoding gene (amoA). The process of simultaneous heterotrophic nitrification and aerobic denitrification (SND) under high salinity was described by measuring the concentration of inorganic N. Halomonas sp. B01 synthesized ectoine under NaCl stress, and the intracellular ectoine concentration increased with increased NaCl concentration in the growth medium. When the NaCl concentration of the medium reached 120 g L−1, the malondialdehyde concentration and SOD activity were significantly increased to 576.1 μg mg−1 and 1.7 U mg−1, respectively. The growth-related and N removal–related enzymes of the strain were active or most active in medium with 30–60 g L−1 NaCl. The amoA of the strain cultured in medium with 60 g L−1 NaCl had the highest mRNA expression abundance. In the N removal medium containing 60 g L−1 NaCl and 2121 mg L−1 NH4+-N, SND by Halomonas sp. B01 was performed over 96 h and the N removal rate reached 98.8%. In addition to the protective mechanism of synthetic compatible solutes, Halomonas sp. B01 had the repair mechanism of SOD for lipid peroxidation. The growth-related and N removal–related enzymes of the strain were most active at a certain salt concentration; amoA also had the highest mRNA expression abundance under high salinity. Halomonas sp. B01 could efficiently perform N removal by SND under high salinity.
中文翻译:
Halomonas sp。的生长和脱氮特性。B01在高盐度下
目前,高盐度含氮废水中微生物处理的氮(N)去除效率相对较低。研究高盐度下中度嗜盐细菌卤单胞菌的脱氮行为和性能对高盐度含氮废水的微生物处理具有重要意义。Halomonas sp。的响应机制。通过测量相容的溶质外啡肽浓度和超氧化物歧化酶(SOD)活性来研究B01对高渗透压的胁迫。通过测量生长相关酶和氮去除相关酶的活性以及氨单加氧酶编码基因(amoA)的mRNA表达丰度,来评估菌株在生长和氮去除过程中的耐盐性。高盐度下同时进行异养硝化和好氧反硝化(SND)的过程通过测量无机N. Halomonas sp。B01在NaCl胁迫下合成了ectoine,并且在生长培养基中,随着NaCl浓度的增加,细胞内ectoine的浓度增加。当培养基的NaCl浓度达到120 g L-1时,丙二醛浓度和SOD活性分别显着增加至576.1μgmg-1和1.7 U mg-1。在含有30–60 g L-1 NaCl的培养基中,该菌株的生长相关酶和N去除相关酶最为活跃。在含有60 g L-1 NaCl的培养基中培养的菌株amoA的mRNA表达量最高。在含有60 g L-1 NaCl和2121 mg L-1 NH4 + -N的N去除介质中,Halomonas sp。B01历时96 h,N去除率达到98.8%。除了合成相容性溶质的保护机制外,Halomonas sp.。B01具有SOD对脂质过氧化的修复机制。在一定的盐浓度下,菌株的生长相关酶和氮去除相关酶最为活跃。在高盐度下,amoA也具有最高的mRNA表达丰度。Halomonas sp。B01可以在高盐度下通过SND有效地去除N。在高盐度下,amoA也具有最高的mRNA表达丰度。Halomonas sp。B01可以在高盐度下通过SND有效地去除N。在高盐度下,amoA也具有最高的mRNA表达丰度。Halomonas sp。B01可以在高盐度下通过SND有效地去除N。
更新日期:2020-04-18
中文翻译:
Halomonas sp。的生长和脱氮特性。B01在高盐度下
目前,高盐度含氮废水中微生物处理的氮(N)去除效率相对较低。研究高盐度下中度嗜盐细菌卤单胞菌的脱氮行为和性能对高盐度含氮废水的微生物处理具有重要意义。Halomonas sp。的响应机制。通过测量相容的溶质外啡肽浓度和超氧化物歧化酶(SOD)活性来研究B01对高渗透压的胁迫。通过测量生长相关酶和氮去除相关酶的活性以及氨单加氧酶编码基因(amoA)的mRNA表达丰度,来评估菌株在生长和氮去除过程中的耐盐性。高盐度下同时进行异养硝化和好氧反硝化(SND)的过程通过测量无机N. Halomonas sp。B01在NaCl胁迫下合成了ectoine,并且在生长培养基中,随着NaCl浓度的增加,细胞内ectoine的浓度增加。当培养基的NaCl浓度达到120 g L-1时,丙二醛浓度和SOD活性分别显着增加至576.1μgmg-1和1.7 U mg-1。在含有30–60 g L-1 NaCl的培养基中,该菌株的生长相关酶和N去除相关酶最为活跃。在含有60 g L-1 NaCl的培养基中培养的菌株amoA的mRNA表达量最高。在含有60 g L-1 NaCl和2121 mg L-1 NH4 + -N的N去除介质中,Halomonas sp。B01历时96 h,N去除率达到98.8%。除了合成相容性溶质的保护机制外,Halomonas sp.。B01具有SOD对脂质过氧化的修复机制。在一定的盐浓度下,菌株的生长相关酶和氮去除相关酶最为活跃。在高盐度下,amoA也具有最高的mRNA表达丰度。Halomonas sp。B01可以在高盐度下通过SND有效地去除N。在高盐度下,amoA也具有最高的mRNA表达丰度。Halomonas sp。B01可以在高盐度下通过SND有效地去除N。在高盐度下,amoA也具有最高的mRNA表达丰度。Halomonas sp。B01可以在高盐度下通过SND有效地去除N。