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Collapsin Response Mediator Protein 2, a Potential Therapeutic Target in Temporal Lobe Epilepsy
Neurochemical Journal ( IF 0.5 ) Pub Date : 2019-06-06 , DOI: 10.1134/s1819712419020144
Xue Wang , Wuqiong Zhang , Jiaai Li , Miaomiao Yu , Ming Dong , Hongmei Meng

Temporal lobe epilepsy (TLE) is the most prevalent subtype of epilepsy in humans and can easily develop into refractory epilepsy. The development of TLE is a complex process involving acute insults, a latent period, and chronic recurrent seizures. The latent period could provide an opportunity for intervention if molecular targets were to be identified. Collapsin response mediator protein 2 (CRMP2) was recently found to be involved in the mechanism of epileptogenesis. However, the results were inconsistent. We aimed to determine if CRMP2 is differentially expressed in rats following status epilepticus (SE), and to validate the expression of CRMP2 during the latent period. We used kainic acid (KA) as the initial insult to induce SE in rats and applied two-dimensional gel electrophoresis (2D-DIGE) coupled with mass spectrometry (MALDI-TOF/TOF) to search for aberrant protein expression in the hippocampus. Western blots were used to confirm the expression level of proteins. Protein extracts of the hippocampus from the sham and the KA groups were separated by 2D-DIGE; 17 protein spots on the gel, representing 10 unique proteins, were found to be significantly different between the KA and sham groups. Among those, CRMP2 was significantly up-regulated. Results of western blot analysis confirmed the increased CRMP2 levels in the KA group. Our findings indicate that the increase in CRMP2 during the latent period is possibly involved in the mechanism of epileptogenesis and is expected to be a novel therapeutic target.

中文翻译:

胶原蛋白介导蛋白2,颞叶癫痫的潜在治疗靶点。

颞叶癫痫(TLE)是人类中最普遍的癫痫亚型,可轻易发展为难治性癫痫。TLE的发展是一个复杂的过程,涉及急性损伤,潜伏期和慢性反复发作。如果要确定分子靶标,则潜伏期可以提供干预的机会。最近发现胶原蛋白应答介导蛋白2(CRMP2)参与了癫痫发生的机制。但是,结果不一致。我们旨在确定在癫痫持续状态(SE)后大鼠中CRMP2是否差异表达,并验证潜伏期CRMP2的表达。我们使用海藻酸(KA)作为诱导大鼠SE的最初侮辱,并应用了二维凝胶电泳(2D-DIGE)和质谱(MALDI-TOF / TOF)来寻找海马中异常的蛋白表达。Western印迹用于确认蛋白质的表达水平。通过2D-DIGE分离假手术组和KA组海马的蛋白质提取物;发现凝胶上的17个蛋白质斑点(代表10种独特的蛋白质)在KA组和假手术组之间存在显着差异。其中,CRMP2明显上调。蛋白质印迹分析的结果证实了KA组中CRMP2水平的升高。我们的发现表明,潜伏期CRMP2的增加可能与癫痫发生的机制有关,并有望成为新型治疗靶点。
更新日期:2019-06-06
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