A novel multistage water-based process was evaluated for the production of sap and other liquid fractions from frozen Sargassum muticum in an attempt to propose an integral and yearly-around utilization of this invasive alga. Three aqueous extracts including (i) the defrosting liquid phase, (ii) the sap liquid obtained by pressing and (iii) the autohydrolysis extract from the solid residue remaining after pressing were characterized for yield, phenolic content, reducing power and antiradical properties. Alginate precipitation and a further liquid–liquid extraction stage using ethyl acetate were assayed to concentrate the phenolic fraction. Alginate exhibited similar viscoelastic features to those commercial available. The aqueous extracts contained 3–12 g phloroglucinol equivalents (PGE)/100 g extract, whereas the ethyl acetate extracts contained up to 55 g PGE/100 g extract and 1 g was as potent ABTS+ radical scavenger as 0.5 g Trolox. The aqueous phase produced by autohydrolysis of the solids were cytotoxic against HCT 116 cells (IC₅₀ < 50 µg mL) without noxious effect on normal cells.

中文翻译：

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冷冻鼠尾草含水部分的抗氧化和抗肿瘤特性

评价了一种新型的多阶段水基工艺，用于从冷冻的Sargassum muticum生产汁液和其他液体馏分试图提出这种入侵藻类的全年综合利用方法。对三种含水提取物进行了表征：收率，酚含量，还原力和抗自由基性能，所述三种含水提取物包括（i）除霜液相，（ii）通过压榨获得的树液和（iii）来自在压榨后剩余的固体残余物中的自水解提取物。分析了藻酸盐的沉淀和进一步的使用乙酸乙酯的液-液萃取阶段，以浓缩酚类馏分。海藻酸盐表现出与可商购的那些相似的粘弹性特征。水性提取物含有3–12 g间苯三酚当量（PGE）/ 100 g提取物，而乙酸乙酯提取物则含有多达55 g PGE / 100 g提取物，而1 g的ABTS +自由基清除剂的效力与0.5 g Trolox相同。₅₀  <50 µg mL）对正常细胞无有害影响。