This study investigates the effects of temperature (50–80 °C), time (10–60 min), carob-to-solvent ratio (2–8 g/40 mL), and solvent concentration (0–50 ethanol/water%, v/v) on microwave-assisted extraction of d-pinitol compound from carob fruit with optimization using Box–Behnken design. The optimal conditions for maximum d-pinitol yield (64.16 g/kg dry sample) include 50 °C temperature, 5.6 g carob/40 mL solvent, water used as a solvent, and an extraction time of 10 min. The fitness of the model was determined by ANOVA analysis with a high coefficient (R² = 0.9057). Extraction was performed under optimum conditions for model validation, and 63.89 g/kg dry sample of d-pinitol extraction was achieved. In addition, the effects of the same variables on the total phenolic (TP) content of MEA extraction of carob were also investigated. Extraction efficiency of 426 g/kg was obtained under the optimum conditions determined for TP (80 °C, 8 g of carob, 50% ethanol, and 10 min), but it was observed that TP content decreased to 49.7 g/kg under optimum conditions determined for d-pinitol. Sucrose, glucose, and fructose sugar contents of the extract were determined only for optimum conditions for d-pinitol, and the fructose content was found to be the lowest. Determining the d-pinitol, TP, and sugar contents of the extract in optimum condition confirms the direct usability of the extract, because only water is used as a solvent. Conventional extraction method was carried out at 50 °C for control purposes, yielding 42.83 g/kg of d-pinitol, thereby supporting the effect of the microwave on extraction.

中文翻译：

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微波辅助从角豆树中提取D-松醇的用途：Box–Behnken设计的应用

这项研究调查了温度（50–80°C），时间（10–60分钟），角豆子与溶剂的比例（2–8 g / 40 mL）和溶剂浓度（0–50乙醇/水％）的影响，v / v），采用Box–Behnken设计，通过微波辅助从角豆果实中提取d-松醇化合物。d-松醇最大产量（64.16 g / kg干样品）的最佳条件包括50°C温度，5.6 g角豆树/ 40 mL溶剂，用作溶剂的水以及10分钟的提取时间。通过ANOVA分析以高系数（R ²  = 0.9057）确定模型的适用性。在最佳条件下进行提取以进行模型验证，然后以63.89 g / kg的干d样品进行提取。-松醇提取。此外，还研究了相同的变量对MEA提取角豆的总酚（TP）含量的影响。在为TP确定的最佳条件下（80°C，8 g角豆树，50％乙醇和10分钟），提取效率为426 g / kg，但观察到在最佳条件下TP含量降至49.7 g / kg确定d-松醇的条件。仅针对d-松醇的最佳条件测定提取物中的蔗糖，葡萄糖和果糖含量，发现果糖含量最低。确定d在最佳条件下，提取物中的松油醇，TP和糖含量证实了提取物的直接可用性，因为仅使用水作为溶剂。为了控制目的，在50℃下进行常规提取方法，得到42.83g / kg的d-松醇，从而支持微波对提取的影响。