Abstract

A simple dual analyte fluorescein-based probe (PF3-Glc) was synthesised containing β-glucosidase (β-glc) and hydrogen peroxide (H₂O₂) trigger units. The presence of β-glc, resulted in fragmentation of the parent molecule releasing glucose and the slightly fluorescent mono-boronate fluorescein (PF3). Subsequently, in the presence of glucose oxidase (GOx), the released glucose was catalytically converted to d-glucono-δ-lactone, which produced H₂O₂ as a by-product. The GOx-produced H₂O₂, resulted in classic H₂O₂-mediated boronate oxidation and the release of the highly emissive fluorophore, fluorescein. This unique cascade reaction lead to an 80-fold increase in fluorescence intensity.

中文翻译：

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双酶激活的荧光素荧光探针

摘要

合成了一个简单的基于双分析物荧光素的探针（PF3-Glc），其中包含β-葡萄糖苷酶（β- glc）和过氧化氢（H ₂ O ₂）触发单元。β- glc的存在会导致母体分子断裂，从而释放葡萄糖和微荧光的单硼酸酯荧光素（PF3）。随后，在葡萄糖氧化酶（GOx）的存在下，释放的葡萄糖被催化转化为d-葡萄糖醛酸-δ-内酯，其产生副产物H ₂ O ₂。GOx生产的H ₂ O ₂，导致经典的H ₂ O ₂介导的硼酸酯氧化和高发射荧光团荧光素的释放。这种独特的级联反应导致荧光强度增加80倍。