Drought stress modulates the expression levels of numerous stress responsive genes which are not yet independently characterized in detail. Isolation and molecular characterization of the novel genes is necessary to understand plant stress physiology and to engineer stress resistant crops. In this study, two drought responsive genes, wheat cold-regulated gene 410c (WCOR410C) and Triticum aestivum Ring Finger 1 (TaRF1), previously implicated in protective roles were selected for characterization. Four bread wheat cultivars (CV) were analyzed for their response towards drought. CV such as NARC and Td-1 appeared as more drought tolerant with less drop in relative water content. Moreover, upon exposure to dehydration stress, differential expression was observed for WCOR410C, a dehydrin gene. However, cultivars tolerance emerged as a function of drought intensity. TaRF1, a ubiquitin ligase, showed drought dependent fourth intron retention in the cultivar Td-1 illustrating the effect of drought stress on the spilceosomal machinery. It is suggested that splicing events giving rise to transcriptome/proteome diversity might play dynamic role in plant’s adaptation to drought stress. Additionally, structural models were predicted for dehydrin domain of WCOR410C and RING-finger domain of TaRF1.

中文翻译：

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小麦中两个新的干旱反应基因的表征

干旱胁迫调节了许多尚未独立表征的胁迫响应基因的表达水平。新基因的分离和分子表征对于了解植物逆境生理和设计抗逆性作物非常必要。在这项研究中，两个干旱响应基因，小麦冷调节基因410c（WCOR410C）和小麦无名指1（Ta选择RF1）（以前与保护角色有关）进行表征。分析了四个面包小麦品种（CV）对干旱的反应。像NARC和Td-1这样的CV表现出更强的耐旱性，相对含水量下降较少。此外，暴露于脱水胁迫下，观察到脱水蛋白基因WCOR410C的差异表达。然而，品种抗性随干旱强度而变化。钽RF1是一种泛素连接酶，在品种Td-1中表现出干旱依赖性的第四内含子保留，说明干旱胁迫对有毛球菌的作用。建议剪接事件产生转录组/蛋白质组多样性可能在植物对干旱胁迫的适应中起动态作用。另外，预测了WCOR410C的脱水蛋白结构域和Ta RF1的RING-指结构域的结构模型。