Cancer is known to be a fierce disease that causes a large percentage of the deaths worldwide. The common cancer treatments; chemotherapy, radiotherapy and surgery are known for their severe side effects; therefore scientists are working on finding solutions to reduce these drawbacks. One of these treatment systems is the sustained released drugs formulations, these systems depend on the encapsulation of the chemotherapy within an emulsifying agent, in order to obtain a slow drug release of low doses over long time intervals. In this study, the anti-cancer effects of free and encapsulated sinapic acid was tested against lung (A549), and colon (CaCo2) cancer cell lines, along with normal fibroblast cells (HFB4) as a negative control. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was performed for IC50 evaluation, also cell cycle assay was performed to detect cell cycle arrest status and related anti-apoptotic and pro-apoptotic; Blc-2, BAX, and P53 gene profile fold changes post cellular treatment. Data recorded revealed that encapsulated SA showed a lower toxicity than the free form to both cell lines and also to the normal cells. The cell cycle analysis showed a cell cycle arrest at the G2/M phase post cell treatment with the free and encapsulated sinapic acid accompanied with up regulation of Bax and P53 and a down regulation of Blc-2 genes in both cell lines. The data suggest a promising anti-cancer and anti-proliferative potential of free and encapsulated sinapic acid. Also they show that the anti-cancer effect of free and encapsulated sinapic acid is quite close.

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组织病理学和遗传学改变证明了游离和纳米囊状芥子酸的抗癌潜力

众所周知，癌症是一种猛烈的疾病，在全世界范围内造成很大比例的死亡。常见的癌症治疗方法；化学疗法，放射疗法和手术以其严重的副作用而著称；因此，科学家们正在努力寻找解决方案，以减少这些弊端。这些治疗系统之一是持续释放的药物制剂，这些系统取决于将化学疗法包封在乳化剂中，以便在长时间间隔内获得低剂量的缓慢药物释放。在这项研究中，测试了游离的和封装的芥子酸对肺癌（A549）和结肠癌（CaCo2）癌细胞系以及正常成纤维细胞（HFB4）的抗癌作用，作为阴性对照。进行了MTT（3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物）测定，以进行IC50评估，还进行了细胞周期测定以检测细胞周期停滞状态以及相关的抗凋亡和促凋亡。细胞治疗后，Blc-2，BAX和P53基因谱折叠发生变化。记录的数据表明，包封的SA对两种细胞系以及正常细胞的毒性均低于游离形式。细胞周期分析显示，在游离和包封的芥子酸处理后，在G2 / M期的细胞周期停滞，同时在两种细胞系中伴随着Bax和P53的上调和Blc-2基因的下调。数据表明游离和包封的芥子酸具有良好的抗癌和抗增殖潜力。他们还表明，游离和封装的芥子酸的抗癌作用非常接近。