A novel cellobiohydrolase (CBH)-generating fungi have been isolated and categorized as Schizophyllum commune KMJ820 based on morphology and rDNA gene sequence. Cellulose powder was used as carbon source, the total enzyme activity was 11.51 U/ml is noted; which is among the highest amounts of CBH-generating microbes studied. CBH have been purified to homogenize, with pursual of serial chromatography using S. commune supernatants and two different CBHs were found; CBH 1 and 2. The filtered CBHs showed greater activity (V_max = 51.4 and 20.8 U/mg) in contrast to CBHs from earlier studies. The MW (molecular weights) of S. commune CBH 1 and 2 were verified to be approximately 50 kDa and 150 kDa, respectively, by size exclusion chromatography. Even though CBHs have been evaluated from other sources, but S. commune CBH is prominent in comparison to other CBHs by its high enzyme activity.

中文翻译：

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裂殖酵母KMJ820的纤维二糖水解酶的表征

根据形态和rDNA基因序列，已分离出一种新型的纤维二糖水解酶（CBH）产生真菌，并将其归类为Schizophyllum commune KMJ820。纤维素粉为碳源，总酶活力为11.51 U / ml。这是研究产生的CBH产生量最高的微生物之一。纯化CBH，使其匀浆，然后使用S. com.une上清液进行连续色谱，发现了两种不同的CBH 。CBH 1和2。 与早期研究的CBH相比，过滤后的CBH表现出更高的活性（V _max = 51.4和20.8 U / mg）。的MW（分子量）S.公社通过尺寸排阻色谱法证实CBH 1和2分别约为50 kDa和150 kDa。即使的CBH已经从其他来源评价，但S.公社CBH是突出在由它的高酶活性，相较于其他的CBH。