The implementation of electron beam radiation coupled with the use of probiotics is one of the newest food processing technologies that may be used to ensure food safety and improve shelf life of food products. The purpose of this study was to evaluate the effect of 50–150-Gy electron beam irradiation on the antimicrobial activity of the putative probiotic strain Lactobacillus rhamnosus Vahe. Low-dose electron beam irradiation of lactobacilli cells was performed using the Advanced Research Electron Accelerator Laboratory’s electron accelerator, and the agar well diffusion method and Verhulst logistic function were used to evaluate the effect of radiation on anti–Klebsiella pneumoniae activity of the cell free supernatant of L. rhamnosus Vahe cells in vitro. Our results suggest that 50–150-Gy electron beam irradiation decreases the viability of the investigated lactobacilli, but does not significantly change the probiotic’s activity against K. pneumoniae. Results indicate that the combined use of irradiation and L. rhamnosus Vahe might be suggested for non-thermal food sterilizing technologies.

中文翻译：

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结合使用eBeam辐射和潜在的益生菌鼠李糖乳杆菌Vahe来控制食源性肺炎克雷伯菌

电子束辐射的实施与益生菌的结合是最新的食品加工技术之一，可用于确保食品安全和改善食品的保质期。这项研究的目的是评估50-150-Gy电子束辐射对推定的益生菌鼠李糖乳杆菌Vahe的抗菌活性的影响。使用高级研究电子加速器实验室的电子加速器对乳酸菌细胞进行低剂量电子束照射，并采用琼脂阱扩散法和Verhulst logistic函数评估辐射对无细胞上清液抗肺炎克雷伯菌活性的影响鼠李糖乳杆菌Vahe细胞的体外培养。我们的结果表明，50–150-Gy电子束辐照会降低所研究的乳酸杆菌的活力，但不会显着改变益生菌对肺炎克雷伯菌的活性。结果表明，辐射和鼠李糖乳杆菌Vahe的联合使用可能被建议用于非热食品灭菌技术。