Floral transitions in continuous-flowering Rosa chinensis ‘Old Blush’ and once-flowering R. odorata var. gigantea were analyzed using comparative transcriptome. In total, 3208 and 5825 species-specific unigenes were identified in the transcriptome profiles of ‘Old Blush’ and R. odorata var. gigantea, respectively. Furthermore, these species-specific unigenes were annotated by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis, and the Mercator web tool was used to mine vital metabolic processes. The contents of soluble sugar and starch in ‘Old Blush’ were higher than those in R. odorata var. gigantea at each stage during the floral transition process. The soluble sugar content was negatively correlated with the starch content in ‘Old Blush’. Therefore, we concluded that the vegetative-to-reproductive phase transition of ‘Old Blush’ was largely controlled by carbon metabolism, especially trehalose. In contrast, the floral transition of R. odorata var. gigantea was potentially mediated by low temperatures (vernalization) and the hexose-mediated miR156 pathway.

中文翻译：

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物种特异性基因解释了连续开花和曾经开花的玫瑰之间的花期过渡差异

连续开花的Rosa chinensis'Old Blush'和曾经开花的R. odorata var的花期过渡。使用比较转录组分析了巨大花。在“ Old Blush”和R. odorata var的转录组谱中，总共鉴定出3208和5825个物种特异性单基因。gigantea。此外，这些物种特定的单基因由基因本体论和《京都议定书》基因与基因组富集分析注释，并且使用Mercator网络工具挖掘重要的代谢过程。'Old Blush'中可溶性糖和淀粉的含量高于R. odorata var。巨大的在花艺过渡过程的每个阶段。“ Old Blush”中的可溶性糖含量与淀粉含量呈负相关。因此，我们得出的结论是“ Old Blush”从营养到生殖的相变在很大程度上受碳代谢，尤其是海藻糖的控制。相反，R。odorata var的花期过渡。gigantea可能是由低温（春化作用）和己糖介导的miR156途径介导的。