For a feasible microalgae biodiesel, increasing lipid productivity is a key parameter. An important cultivation parameter is light wavelength (λ). It can affect microalgal growth, lipid yield, and fatty acid composition. In the current study, the mixture design was used as an alternative to model the influence of the λ on the Dunaliella salina lipid productivity. The illumination was considered to be the mixture of different λ (the light colors blue, red, and green). All experiments were performed with and without sodium acetate (4 g/L), as carbon source, allowing the identification of the impact of the cultivation regimen (autotrophic or mixotrophic). Without sodium acetate, the highest lipid productivity was obtained using blue and red light. The use of mixotrophic cultivations significantly enhanced the results. The optimum obtained result was mixotrophic cultivation under 65% blue and 35% green light, resulting in biomass productivity of 105.06 mgL⁻¹day⁻¹, a lipid productivity of 53.47 mgL⁻¹day⁻¹, and lipid content of 50.89%. The main fatty acids of the oil obtained in this cultivation were oleic acid (36.52%) and palmitic acid (18.31%).

对于可行的微藻生物柴油，提高脂质生产率是关键参数。重要的培养参数是光波长（λ）。它会影响微藻生长，脂质产量和脂肪酸组成。在当前的研究中，混合物设计被用作模拟λ对杜氏盐藻盐藻脂质生产力的影响的替代方法。照明被认为是不同λ的混合（浅色为蓝色，红色和绿色）。所有实验均在有和没有乙酸钠（4 g / L）作为碳源的条件下进行，从而可以确定培养方案的影响（自养或混养）。没有乙酸钠，使用蓝光和红光可获得最高的脂质生产率。混合营养栽培的使用显着增强了结果。最佳的结果是在65％的蓝光和35％的绿光下进行营养混合培养，生物量生产力为105.06 mgL ^-1 day ^-1，脂质生产力为53.47 mgL ^-1 day ^-1，脂质含量为50.89％。在该培养中获得的油的主要脂肪酸为油酸（36.52％）和棕榈酸（18.31％）。