Colla corii asini (CCA) made from donkey-hide has been widely used as a traditional animal-based Chinese medicine. Chondroitin sulfate (CS), dermatan sulfate (DS) and hyaluronic acid (HA) are structurally complex classes of glycosaminoglycans (GAGs) that have been implicated in a wide range of biological activities. However, their possible structural characteristics in CCA are not clear. In this study, GAG fractions containing CS/DS and HA were isolated from CCA and their disaccharide compositions were analyzed by high sensitivity liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-MS-ITTOF). The result showed that CS/DS/HA disaccharides were detected in the three lower salt fractions from anion-exchange chromatography. The sulfation patterns and densities of CS/DS chains in these fractions differed greatly, while HA chains varied in their chain lengths. The quantitative analysis first revealed that the amount of GAGs in CCA varied significantly in total and in each fraction. This novel structural information could help clarify the possible involvement of these polysaccharides in the biological activities of CCA.

中文翻译：

---

液相色谱-电喷雾离子阱质谱法分析Colla corii asini中糖胺聚糖的结构

由驴皮制成的Colla corii asini（CCA）已被广泛用作基于动物的传统中药。硫酸软骨素（CS），硫酸皮肤素（DS）和透明质酸（HA）是结构复杂的糖胺聚糖（GAG），涉及多种生物活性。但是，它们在CCA中可能的结构特征尚不清楚。在这项研究中，从CCA中分离出含有CS / DS和HA的GAG馏分并用高灵敏度液相色谱-离子阱/飞行时间质谱（LC-MS-ITTOF）分析了它们的二糖成分。结果表明，阴离子交换色谱法在三个较低的盐级分中检测到CS / DS / HA二糖。这些级分的CS / DS链的硫酸化模式和密度差异很大，而HA链的链长却有所不同。定量分析首先显示，CCA中GAG的总量和每个组分中的含量差异显着。这种新的结构信息可能有助于阐明这些多糖可能与CCA的生物活性有关。