SummaryThe objective of this study is to compare aneuploidy rates between three distinct areas of the human trophectoderm: mural, polar and a region in between these two locations termed the ‘mid’ trophectoderm. This is a cohort study on in vitro fertilization (IVF) patients undergoing comprehensive chromosome screening at the blastocyst stage at a private IVF clinic. All embryos underwent assisted hatching on day 3 with blastocyst biopsy and comprehensive chromosome screening. Biopsied blastocysts were divided into three groups depending on which area (polar, mid, or mural) of the trophectoderm was protruding from the zona pellucida and biopsied. Aneuploidy rates were significantly higher with cells from the polar region of the trophectoderm (56.2%) compared with cells removed from the mural region of the trophectoderm (30.0%; P = 0.0243). A comparison of all three areas combined also showed a decreasing trend, but this did not reach clinical significance, polar (56.2%), mid (47.4%) and mural trophectoderm (30.0%; P = 0.1859). The non-concordance demonstrated between polar and mural trophectoderm can be attributed to biological occurrences including chromosomal mosaicism or procedural differences between embryologists.

中文翻译：

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极地、中层和壁滋养外胚层之间非整倍体率的初步评估

摘要本研究的目的是比较人类滋养外胚层三个不同区域之间的非整倍体率：壁、极地和这两个位置之间的区域，称为“中”滋养外胚层。这是一项关于体外受精 (IVF) 患者在私人 IVF 诊所在囊胚阶段接受全面染色体筛查。所有胚胎都在第 3 天进行了辅助孵化，并进行了囊胚活检和全面的染色体筛查。根据滋养外胚层从透明带突出的区域（极地、中部或壁）将活检囊胚分为三组并进行活检。与来自滋养外胚层壁区的细胞（30.0%；磷= 0.0243)。所有三个区域的比较也显示出下降趋势，但这并没有达到临床意义，极地（56.2%）、中间（47.4%）和附壁滋养外胚层（30.0%；磷= 0.1859）。极地和壁滋养外胚层之间表现出的不一致可归因于生物学现象，包括染色体嵌合或胚胎学家之间的程序差异。