Integration of HBV DNA into host chromosomes was found in most of the patients with chronic hepatitis B (CHB). In this study, using inverse nested PCR (invPCR), we found the integration site chrX: 111,009,033, which inserted into the p21-activated kinase 3 (PAK3) gene in HepG2.2.15 cells. The viral-human chimeric transcripts were also observed and, significant differences of the copy numbers of integration site chrX: 111,009,033 (P = 0.012) and intra-cell HBV DNA levels (P = 0.027) were found between the cells with and without H₂O₂ treatment, respectively. This study may provide a novel insight into the elucidation of etiology of HBV integration.

在大多数慢性乙型肝炎（CHB）患者中发现了HBV DNA整合入宿主染色体。在这项研究中，使用反向嵌套PCR（invPCR），我们发现了整合位点chrX：111,009,033，该位点插入了HepG2.2.15细胞中p21激活的激酶3（PAK3）基因中。还观察到了病毒-人的嵌合转录本， 在有和没有H ₂的细胞之间，整合位点chrX的拷贝数有明显差异：111,009,033（P  = 0.012）和细胞内HBV DNA水平（P = 0.027）。O ₂分别处理。这项研究可能为阐明HBV整合的病因提供新的见解。