This study aimed to explore involvement of oxidative stress in sterigmatocystin (STC) toxicity in male Wistar rats. Animals were orally treated with a single STC dose (10, 20 and 40 mg/kg b.w.). Short-term treatment resulted in moderate oxidative stress determined by a significant increase of malondialdehyde (MDA; all STC doses) and catalase (CAT; 10 mg/kg b.w.) in plasma, a decrease of glutathione peroxidase (GPx; 20 and 40 mg/kg b.w.) in the liver, and increase of MDA and superoxide dismutase (SOD) in kidneys (all STC doses). Heat shock protein (Hsp27 and Hsp70) expression was determined by Western blotting in rat liver and kidneys. Hsp27 expression was downregulated by STC, particularly in the liver (40 mg/kg b.w.). The lowest STC dose elevated the expression of Hsp70 in both liver and kidneys, while an increase in STC doses restored Hsp70 expression to control. Alterations in expressions of Hsp27 and Hsp70 could be only partially associated with oxidative stress. STC provoked a significant DNA damage in both liver and kidneys (alkaline comet assay), but the liver was more affected by a broader spectrum of DNA lesions. Oxidative DNA damage (hOGG1-modified comet assay) contribute to the overall mechanism of STC-induced DNA damage in both organs, but kidneys in general seem to be more susceptible to oxidative stress upon short-term exposure to sublethal doses of STC.

中文翻译：

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短期口服治疗后，去角质囊藻毒素可适度诱导雄性Wistar大鼠氧化应激。

这项研究旨在探讨氧化应激参与雄性Wistar大鼠的stercysttocystin（STC）毒性。用单次STC剂量（10、20和40 mg / kg bw）对动物进行口服治疗。短期治疗导致中等程度的氧化应激，这取决于血浆中丙二醛（MDA;所有STC剂量）和过氧化氢酶（CAT; 10 mg / kg bw）的显着增加，谷胱甘肽过氧化物酶（GPx; 20和40 mg /肝脏中的MDA和超氧化物歧化酶（SOD）升高（所有STC剂量）。通过蛋白质印迹法测定大鼠肝脏和肾脏中的热休克蛋白（Hsp27和Hsp70）的表达。Hsp27表达被STC下调，尤其是在肝脏中（40 mg / kg bw）。最低的STC剂量可提高肝和肾中Hsp70的表达，而增加STC剂量可将Hsp70表达恢复至正常水平。Hsp27和Hsp70表达的改变可能只与氧化应激部分相关。STC在肝脏和肾脏中都引起了明显的DNA损伤（碱性彗星试验），但是肝脏受到更大范围DNA损伤的影响更大。氧化性DNA损伤（hOGG1修饰的彗星测定法）有助于STC诱导两个器官中DNA损伤的整体机制，但肾脏通常似乎在短期暴露于亚致死剂量的STC时更容易受到氧化应激。但是肝脏受到更大范围DNA损伤的影响更大。氧化性DNA损伤（hOGG1修饰的彗星测定法）有助于STC诱导两个器官中DNA损伤的整体机制，但肾脏通常似乎在短期暴露于亚致死剂量的STC时更容易受到氧化应激。但是肝脏受到更大范围DNA损伤的影响更大。氧化性DNA损伤（hOGG1修饰的彗星测定法）有助于STC诱导两个器官中DNA损伤的整体机制，但肾脏通常似乎在短期暴露于亚致死剂量的STC时更容易受到氧化应激。