A previous animal study by our group found that sleep deprivation during preimplantation was associated with decreased pregnancy maintenance. Given its impact on human society, we aimed in the current study to assess whether sleep deprivation affects blastocyst gene expression and/or the implantation process. For this, pregnant mice (gestational day 0 [GD 0]) were assigned into paradoxical sleep deprivation (SD, 72 hr; multiple platform method) and, a control (CT) group. Animals were euthanized on GD 3.5 and blood, uterus (embryos) and fallopian tube were collected. Then, 89% of CT presented blastocysts in the uterus versus 25% from SD group. Compared to CT, SD presented lighter relative uterus weight, increased plasma concentrations of corticosterone and testosterone, decreased concentrations of progesterone and luteinizing hormone, but no statistical differences in plasma concentrations of 17β‐estradiol and follicle stimulating hormone. There were no differences in uterus and blastocyst gene expression related to embryo implantation and development, and no alteration in blastocysts global DNA methylation. Considering this, the decreased pregnancy maintenance after sleep deprivation seems not to be associated with implantation losses or developmental problems related to the blastocysts. It is likely that complications in morula development and/or its movement through the fallopian tubes affect the pregnancy rate, since only 25% of SD females presented a blastocyst on the GD 3.5. In fact, three out of four females without blastocysts in the uterus presented morula in the fallopian tubes due to a phase delay. Additionally, we suggest that the observed hormonal changes may play a role in this outcome.

中文翻译：

---

睡眠不足会影响桑在子宫内的到达，从而降低生殖能力。

我们小组先前的一项动物研究发现，植入前的睡眠不足与妊娠维持率降低有关。鉴于其对人类社会的影响，我们在本研究中旨在评估睡眠剥夺是否会影响胚泡基因表达和/或植入过程。为此，将妊娠小鼠（妊娠第0天[GD 0]）分为反常睡眠剥夺（SD，72小时；多平台法）和对照组（CT）。用GD 3.5对动物实施安乐死，并收集血液，子宫（胚胎）和输卵管。然后，有89％的CT表现为子宫内的胚泡，而SD组则为25％。与CT，SD相比表现出较轻的相对子宫重量，皮质酮和睾丸激素的血浆浓度增加，孕酮和黄体生成激素的浓度降低，但血浆17β-雌二醇和促卵泡激素的浓度无统计学差异。子宫和胚泡基因表达与胚胎植入和发育有关没有差异，胚泡全局DNA甲基化也没有改变。考虑到这一点，睡眠剥夺后减少的妊娠维持似乎与植入丢失或与胚泡有关的发育问题无关。桑发育和/或通过输卵管移动的并发症可能会影响怀孕率，因为只有25％的SD女性在GD 3.5上出现了胚泡。事实上，子宫中没有胚泡的四分之三女性由于相延迟而在输卵管中出现桑。此外，我们建议观察到的荷尔蒙变化可能在这一结果中起作用。