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Nasal administration of gonadotropin releasing hormone (GnRH) elicits sperm production in Fowler’s toads (Anaxyrus fowleri)
BMC Zoology ( IF 1.6 ) Pub Date : 2019-05-22 , DOI: 10.1186/s40850-019-0040-2
Allison R. Julien , Andrew J. Kouba , David Kabelik , Jean M. Feugang , Scott T. Willard , Carrie K. Kouba

Declining amphibian populations around the world necessitate the establishment of captive assurance colonies as a hedge against extinction. For species that are difficult to breed in captivity, assisted reproductive techniques, such as treatment with exogenous hormones, are necessary for successful reproduction. The purpose of this study was to determine whether intranasal administration of a gonadotropin releasing hormone analog (GnRHa) elicits sperm production in anurans. Male Anaxyrus fowleri (n = 15/trt) were nasally administered GnRHa (1, 5, 10, and 20 μg) in phosphate buffered saline (PBS) using a pipette. Spermic urine was collected from animals over an 8 h period. Samples were assessed for sperm presence, motility, and concentration. Treatment of male toads with a PBS control and 1 μg of GnRHa did not elicit sperm production. Nasal administration of GnRHa resulted in sperm production from 60, 93, and 80% of males in the 5, 10, and 20 μg treatment groups, respectively. Sperm motilities averaged 70, 63, and 52% within the 5, 10, and 20 μg treatment groups, respectively, with the highest (p < 0.05) sperm motility observed using 5 μg of GnRHa. Significantly higher sperm concentrations were observed in males treated with 10 μg of GnRHa compared with 5 or 20 μg of GnRHa. Nasal administration of GnRHa was successful in eliciting spermiation from male Anaxyrus fowleri, which typically began less than 3 h after treatment. Nasal administration of GnRHa may provide a novel non-invasive method of hormone delivery for at-risk amphibian species that have low reproductive output.

中文翻译:

鼻腔施用促性腺激素释放激素(GnRH)会引起福勒蟾蜍(Anaxyrus fowleri)产生精子。

世界各地两栖动物数量的下降,有必要建立专属的保证殖民地,以此来防止灭绝。对于难以在繁殖中繁殖的物种,辅助生殖技术(例如用外源激素处理)是成功繁殖所必需的。这项研究的目的是确定鼻腔内促性腺激素释放激素类似物(GnRHa)的给药是否会引起无精子中精子的产生。使用移液管向雄性无水狐(Anaxyrus fowleri)(n = 15 / trt)经磷酸盐缓冲液(PBS)经鼻给予GnRHa(1、5、10和20μg)。在8小时内从动物身上收集精子尿。评估样品的精子存在,运动性和浓度。用PBS对照和1μgGnRHa处理雄性蟾蜍不会引起精子产生。鼻腔施用GnRHa分别导致5、10和20μg治疗组中60%,93%和80%的男性产生精子。在5、10和20μg治疗组中,精子活动率分别平均为70%,63%和52%,使用5μgGnRHa观察到的精子活动率最高(p <0.05)。相比于5或20μgGnRHa,在用10μgGnRHa治疗的雄性中观察到明显更高的精子浓度。鼻腔施用GnRHa成功地引发了雄性Anaxyrus fowleri的精子,通常在治疗后不到3小时开始。鼻腔给予GnRHa可能为生殖低产的高危两栖动物提供一种新型的非侵入性激素输送方法。和20μg治疗组。在5、10和20μg治疗组中,精子活动率分别平均为70%,63%和52%,使用5μgGnRHa观察到的精子活动率最高(p <0.05)。相比于5或20μgGnRHa,在用10μgGnRHa治疗的雄性中观察到明显更高的精子浓度。鼻腔施用GnRHa成功地引发了雄性Anaxyrus fowleri的精子,通常在治疗后不到3小时开始。鼻腔给予GnRHa可能为生殖低产的高危两栖动物提供一种新型的非侵入性激素输送方法。和20μg治疗组。在5、10和20μg治疗组中,精子活动率分别平均为70%,63%和52%,使用5μgGnRHa观察到的精子活动率最高(p <0.05)。相比于5或20μgGnRHa,在用10μgGnRHa治疗的雄性中观察到明显更高的精子浓度。鼻腔施用GnRHa成功地引发了雄性Anaxyrus fowleri的精子,通常在治疗后不到3小时开始。鼻腔给予GnRHa可能为生殖低产的高危两栖动物提供一种新型的非侵入性激素输送方法。相比于5或20μgGnRHa,在用10μgGnRHa治疗的雄性中观察到明显更高的精子浓度。鼻腔施用GnRHa成功地引发了雄性Anaxyrus fowleri的精子,通常在治疗后不到3小时开始。鼻腔给予GnRHa可能为生殖低产的高危两栖动物提供一种新型的非侵入性激素输送方法。相比于5或20μgGnRHa,在用10μgGnRHa治疗的雄性中观察到明显更高的精子浓度。鼻腔施用GnRHa成功地引发了雄性Anaxyrus fowleri的精子,通常在治疗后不到3小时就开始。鼻腔给予GnRHa可能为生殖低产的高危两栖动物提供一种新型的非侵入性激素输送方法。
更新日期:2019-05-22
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