Breast tissue remodeling occurs throughout a women’s life from pre-menstrual to post menopausal time, hence there is a high risk of DNA damage. Estrogen metabolite generates free radical that causes DNA damage leading to cancer cell formation. Moringa oleifera leaf extract contain rich amount of Polyphenolic and Flavonoids compound which trap the free radical and thereby prevent cancer cell progression. Moreover anti-gonadotrophic hormonal activity of the leaf extract slows down the proliferation rate of T47D cell line. Hence our aim of study mainly focused to develop an anticancer drug delivery system using M. oleifera leaf extract. Silver nanoparticle (AgNp) is formed by green synthesis method using M. oleifera leaf extract. Reducing sugar, Flavonoids, Polyphenolic compound, and NADPH dependent dehydrogenase present in M. oleifera leaf extract are responsible for reduction of the silver ion (Ag⁺) followed by formation of metallic silver (Ag⁰). The formation of spherical and rectangular shaped AgNp with average size of 50 nm is reflected with sharp SPR band near 420 nm and from SEM, TEM analysis. The nanoparticle mediated polyvinyl alcohol composite film (nano-rod) is prepared for delivery of the purified anti-neoplastic phyto-compound from leaf extract. The large surface area of AgNp permits its coordination with purified compound molecule by metal ligand bonding. Therefore it provide duel effect, it acts as carrier molecule for phyto-compound and also creates cytotoxicity effect on cancer cells. Single dose treatment on HeLa cell indicates that the composite film is more effective in killing the cancer cell than the purified anti-neoplastic phyto-compound.

中文翻译：

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辣木叶提取物用于银纳米粒子介导的药物递送系统合成的生化估计

从绝经前到绝经后的一生中，乳房组织的重塑都会发生，因此存在DNA受损的高风险。雌激素代谢产物产生自由基，该自由基引起DNA损伤，导致癌细胞形成。辣木叶提取物含有丰富的多酚和类黄酮化合物，可捕获自由基，从而阻止癌细胞的进展。此外，叶提取物的抗性腺激素活性减慢了T47D细胞系的增殖速率。因此，我们的研究目标主要集中在使用油橄榄叶提取物开发抗癌药物递送系统。银绿色颗粒（AgNp）是通过绿色合成方法使用油橄榄（M.oleifera）形成的。叶提取物。油茶叶片提取物中存在的还原糖，类黄酮，多酚化合物和NADPH依赖性脱氢酶负责还原银离子（Ag ⁺），然后形成金属银（Ag ⁰）。平均粒径为50 nm的球形和矩形AgNp的形成在420 nm附近具有清晰的SPR谱带并通过SEM，TEM分析得到反映。制备了纳米粒子介导的聚乙烯醇复合膜（纳米棒），用于从叶提取物中递送纯化的抗肿瘤植物化合物。AgNp的大表面积允许其通过金属配体键与纯化的化合物分子配位。因此，它提供了双重作用，它充当植物化合物的载体分子，并且还对癌细胞产生细胞毒性作用。HeLa细胞的单剂量治疗表明，复合膜比纯化的抗肿瘤植物化合物在杀死癌细胞方面更有效。