Regenerated cellulose can be prepared by treatment with an ionic liquid (IL) and an anti-solvent such as water, which significantly enhances the enzymatic hydrolysis in comparison to crystalline cellulose. The IL-aqueous two-phase system (IL-ATPS) is consisted of IL-condensed top phase and salt-condensed bottom phase, which could be suitable to produce regenerated cellulose with smaller amount of IL. Using IL-ATPS with different pH, the enzymatic saccharification efficiency of crystalline cellulose was determined. The use of 1-allyl-3-methylimidazolium chloride resulted in relatively higher yield of glucose production as compared to 1-butyl-3-methylimidazolium chloride. The IL-ATPS showing optimal pH for cellulase was prepared with mixed salt (NaH₂PO₄/Na₂HPO₄ = 5/1 (wt/wt)), which provide a regenerated cellulose with the pH range of 4.8–4.9 in enzymatic reaction mixture. Using such regenerated cellulose as feed of saccharification, the final yield of glucose was about 70%.

再生纤维素可以通过用离子液体（IL）和抗溶剂（例如水）处理来制备，与结晶纤维素相比，该抗溶剂显着增强了酶促水解作用。IL-水两相系统（IL-ATPS）由IL浓缩的上层相和盐浓缩的下层相组成，可能适合生产IL含量较小的再生纤维素。使用不同pH的IL-ATPS，测定了结晶纤维素的酶促糖化效率。与1-丁基-3-甲基咪唑氯化物相比，使用1-烯丙基-3-甲基咪唑氯化物可产生相对更高的葡萄糖产量。用混合盐（NaH ₂ PO ₄ / Na ₂ HPO制备显示纤维素酶最佳pH的IL-ATPS₄  = 5/1（wt / wt）），可在酶促反应混合物中提供pH范围为4.8-4.9的再生纤维素。使用这种再生纤维素作为糖化进料，葡萄糖的最终产率为约70％。