Abstract

The role of autophagy in the development and progression of hepatocellular carcinoma (HCC) is ambiguous and still little known. Autophagy stimulation may be of exceptional interest in antitumor pharmacotherapy of HCC. Rapamycin and lithium are typical inducers of autophagy. The aim of this study was to compare the level of autophagy in hepatocellular carcinoma-29 (HCC-29) cells after single and combined administration of lithium carbonate and rapamycin. Autolysosomes formation and significant increase of LC3 beta (+)– and LAMP1 (+)– autophagic structures were revealed in HCC-29 cells after lithium carbonate and rapamycin coadministration by transmission electron microscopy and immunofluorescence analysis. Using this combination of drugs may be a promising strategy for HCC chemotherapy, since it will allow the integration of various cellular signaling pathways that regulate autophagy and apoptosis in tumor cells.

中文翻译：

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碳酸锂和雷帕霉素单次或联合给药后在肝细胞癌29中的自噬

摘要

自噬在肝细胞癌（HCC）的发生和发展中的作用尚不明确，尚不清楚。自噬刺激在肝癌的抗肿瘤药物治疗中可能是特别感兴趣的。雷帕霉素和锂是自噬的典型诱导剂。这项研究的目的是比较碳酸锂和雷帕霉素单次或联合给药后肝细胞癌29（HCC-29）细胞的自噬水平。通过透射电子显微镜和免疫荧光分析，碳酸锂和雷帕霉素共同给药后，HCC-29细胞显示了溶酶体形成和LC3β（+）–和LAMP1（+）–自噬结构的显着增加。使用这种药物组合可能是HCC化疗的有前途的策略，