Late leaf spot (LLS) is the major foliar disease, which often occur with rust and cause severe yield losses worldwide in peanut (Arachis hypogaea L.). In order to map and introgress the resistance alleles for LLS into an elite cultivar ICGS 76, the advanced backcross approach was used for identification of quantitative trait locus (QTL). The synthetic amphidiploid ISATGR 278–18 (A. duranensis ICG 8138 × A. batizocoi ICG 13160) which is known to harbor resistance alleles for foliar diseases was used as donor parent to generate AB-QTL population comprising of 164 introgression lines (ILs). A linkage map with 114 microsatellite markers was developed spanning 746.15 cM distance with an average inter-marker distance of 6.55 cM. QTL analysis yielded 14 main-effect QTLs (mQTLs) (explaining up to 38.58% PVE) for LLS and 10 mQTLs (up to 10.99% PVE) for rust. In addition, four epistatic QTLs (eQTLs) (explaining up to 4.97% PVE) were observed for foliar diseases. Furthermore, seven mQTLs and 16 eQTLs were observed for agronomic traits. Several ILs showed high resistance to LLS with agronomic attributes from recurrent parent. This study reports the utilization of the untapped resistance source for LLS from A. batizocoi that was not extensively used earlier in peanut crop improvement.

中文翻译：

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利用合成的二倍体先进回交群体解剖花生对晚叶斑病的抗性

晚期叶斑病（LLS）是主要的叶病，经常发生锈病并导致全世界花生（Arachis hypogaea L.）的严重减产。为了将LLS的抗性等位基因作图并导入到一个优良的栽培品种ICGS 76中，采用了先进的回交方法来鉴定数量性状基因座（QTL）。合成的二倍体ISATGR 278-18（A . duranensis ICG 8138×  A. batizocoiICG 13160）（已知具有针对叶病的抗性等位基因）被用作供体亲本，以生成由164个基因渗入系（IL）组成的AB-QTL群体。建立了具有114个微卫星标记的连锁图，其跨距746.15 cM，平均标记间距离为6.55 cM。QTL分析得出用于LLS的14个主要效应QTL（mQTL）（解释为PVE的38.58％）和针对铁锈的10 mQTL（解释为PVE的10.99％）。此外，还观察到了四个叶面疾病的上位QTL（eQTL）（解释为PVE高达4.97％）。此外，观察到7个mQTL和16个eQTL的农艺性状。几种IL显示出对来自轮回亲本具有农艺学特性的LLS的高抗性。这项研究报告了利用未开发的抗药性来源的巴氏曲霉 尚未在花生作物改良中广泛使用。