Altzre-testing (POCT) applications, its commercialization has been hampered due to complex fabrication processes or the need for additional lab instruments. In this study, tubing-based colourimetric and volumetric enzyme-linked immunosorbent assay (ELISA) methods were developed for application in POCT of cortisol and Salmonella typhimurium, respectively. Different solutions required for each assay were contained in a piece of tubing, forming different aqueous layers that were separated by immiscible organic layers. The assay was carried out by moving antibody-functionalized, solid-phase magnetic particles through the layers with a hand-operated magnet. This method enabled detection of cortisol at a low detection limit of 0.1 ng/mL within 12 min and detection of S. typhimurium down to 10² cfu/sample within 1 h. The performance of the assay methods was comparable to or better than that of conventional ELISA despite the assay systems and methods being extremely simple, thus demonstrating its potential for POCT application.

中文翻译：

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管式固定液相酶联免疫吸附法的发展

在Altzre测试（POCT）应用中，由于复杂的制造过程或需要其他实验室仪器而使其商业化受到阻碍。在这项研究中，开发了基于管的比色法和体积酶联免疫吸附测定（ELISA）方法，分别用于皮质醇和鼠伤寒沙门氏菌的POCT中。每个测定所需的不同溶液包含在一根管中，形成了不同的水层，这些水层被不混溶的有机层隔开。通过使用手动操作的磁体将抗体功能化的固相磁性颗粒移动穿过各层，从而进行了分析。该方法能够在12分钟内以0.1 ng / mL的低检测限检测皮质醇，并检测鼠伤寒沙门氏菌在1小时内降至10 ² cfu /样品。尽管测定系统和方法非常简单，但测定方法的性能可与传统ELISA相当或更好，从而证明了其在POCT应用中的潜力。