Mosquitoes are vectors for diseases damaging human health and thus, there is an urgent need for insecticidal compounds to control their population. The objective of this study was to determined the efficiency from trans-cinnamic acid isolated from Alpinia galanga (Zingiberales: Zingiberaceae) for control of Aedes aegypti (Dipetera: Culicidae), Anopheles dirus B (Dipetera: Culicidae) and Culex quinquefasciatus (Dipetera: Culicidae). Alpinia galanga (Zingiberales:Zingiberaceae) was extracted by soaking in a sequence of solvents (hexane, dichloromethane, ethyl acetate and methanol), and the isolated trans-cinnamic was separated by preparative thin layer chromatography. All crude extracts and isolated trans-cinnamic were evaluated for their control and affect on detoxification enzyme activities of the third-instar larvae of each mosquito species in laboratory conditions. Our results showed that the hexane crude extract had the best control efficiency in all species, particularly in Cx. quinquefasciatus. The trans-cinnamic acid, isolated compound from hexane crude extract showed as active ingredient against third-instar larvae of each mosquito species. Mortality in this case may result from the inhibition of carboxylesterase. These results indicated that A. galanga which had trans-cinnamic acid as active ingredient compound could represent a promising naturally occurring control agent for all three mosquito species. However, this research consider as an initial prospective study, the other side effect on nontarget species need to concerned before used as commercial product.

中文翻译：

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评价高良姜（姜科）提取物和分离的反式对一些蚊子幼虫-肉桂酸

蚊子是破坏人类健康的疾病的媒介，因此迫切需要杀虫化合物来控制其种群。这项研究的目的是确定从高良姜（Alpinia galanga）（Zingiberales：Zingiberaceae）分离得到的反式肉桂酸对埃及伊蚊（Dipetera：Culicidae），狄氏按蚊B（Dipetera：Culicidae）和库克斯库克斯（Qulex quinquefasciatus）（Dipetera：Culicidae）的控制效率。 ）。通过浸入一系列溶剂（己烷，二氯甲烷，乙酸乙酯和甲醇）中提取高良姜（姜科植物：姜科），并且通过制备薄层色谱分离分离的反式肉桂酸酯。在实验室条件下，对所有粗提取物和分离的反式肉桂进行了评估，以控制它们并影响每种蚊子第三龄幼虫的解毒酶活性。我们的结果表明，在所有物种中，特别是在Cx中，己烷粗提物具有最佳的控制效率。quinquefasciatus。从己烷粗提物中分离得到的反肉桂酸化合物显示出作为对抗每种蚊子第三龄幼虫的活性成分。在这种情况下，死亡率可能是由于羧酸酯酶的抑制所致。这些结果表明具有反式肉桂酸作为活性成分化合物的高良曲霉可以代表所有三种蚊子的有希望的天然防治剂。但是，本研究认为这是一项初步的前瞻性研究，