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Characterization of the Different Stages of Biofilm Formation and Antibiotic Susceptibility in a Clinical Acinetobacter baumannii Strain.
Microbial Drug Resistance ( IF 2.6 ) Pub Date : 2020-06-08 , DOI: 10.1089/mdr.2019.0145
Paula Da Cunda 1 , Victoria Iribarnegaray 1 , Romina Papa-Ezdra 2 , Inés Bado 2 , María José González 1 , Pablo Zunino 1 , Rafael Vignoli 2 , Paola Scavone 1
Affiliation  

Acinetobacter baumannii is a relevant opportunistic pathogen, and one of the main microorganisms responsible for outbreaks in nosocomial infections worldwide. Its pathogenicity is mainly due to its resistance to multiple antibiotics and to its ability to form biofilms on abiotic surfaces. The objective of this study was to characterize the biofilm formation cycle of A. baumannii isolated from a patient in a hospital and compare its antibiotic resistance with the planktonic cells. To study biofilm formation, the classical microtiter assay was used, with crystal violet staining and optical density reading to classify the type of biofilm. Also, the effect of gentamicin and colistin on bacterial biofilm was studied with an extra step of antibiotic addition. For the characterization of the different biofilm formation stages, the strain was grown on a coverslip, and the stain was made with a mixture of fluorophores markers to visualize the biofilm with a confocal laser microscope. It was possible to differentiate the A. baumannii biofilm formation stages. Through these observations, it was possible to estimate the time elapsed between each stage. As the strain was susceptible to colistin and gentamicin, both antibiotics were evaluated after the biofilm was formed. Neither antibiotics showed an effect on the eradication of A. baumannii biofilm.

中文翻译:

临床鲍曼不动杆菌菌株中生物膜形成和抗生素敏感性不同阶段的表征。

鲍曼不动杆菌是一种相关的机会病原体,是引起全球医院感染暴发的主要微生物之一。其致病性主要归因于其对多种抗生素的抗性以及在非生物表面上形成生物膜的能力。这项研究的目的是表征鲍曼不动杆菌的生物膜形成周期从一家医院的患者身上分离出来,并将其抗药性与浮游细胞进行比较。为了研究生物膜的形成,使用了经典的微量滴定法,通过结晶紫染色和光密度读数对生物膜的类型进行分类。另外,还通过添加抗生素的额外步骤研究了庆大霉素和粘菌素对细菌生物膜的影响。为了表征不同的生物膜形成阶段,使菌株在盖玻片上生长,并用荧光团标记混合物进行染色,以用共聚焦激光显微镜观察生物膜。可以区分鲍曼不动杆菌生物膜形成阶段。通过这些观察,可以估计每个阶段之间经过的时间。由于该菌株对粘菌素和庆大霉素敏感,因此在形成生物膜后评估了两种抗生素。两种抗生素均未显示出根除鲍曼不动杆菌生物膜的作用。
更新日期:2020-06-08
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