Abstract

The domestic ferret, Mustela putorius furo, is an important mammalian animal model to study human respiratory infection. However, insufficient genomic annotation hampers detailed studies of ferret T cell responses. In this study, we analyzed the published T cell receptor beta (TRB) locus and performed high-throughput sequencing (HTS) of peripheral blood of four healthy adult ferrets to identify expressed V, D, J, and C genes. The HTS data is used as a guide to manually curate the expressed V, D, J, and C genes. The ferret locus appears to be most similar to that of the dog. Like other mammalian TRB loci, the ferret TRB locus contains a library of variable genes located upstream of two D-J-C gene clusters, followed by a (in the ferret non-functional) V gene with an inverted transcriptional orientation. All TRB genes (expressed or not) reported here have been approved by the IMGT/WHO-IUIS nomenclature committee.

中文翻译：

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V，D，J和C基因表达分析指导的雪貂TRB基因座的表征

摘要

国产雪貂，鼬鼬是研究人类呼吸道感染的重要哺乳动物模型。然而，不足的基因组注释妨碍了对雪貂T细胞反应的详细研究。在这项研究中，我们分析了已发表的T细胞受体β（TRB）基因座，并对四个健康成年雪貂的外周血进行了高通量测序（HTS），以鉴定表达的V，D，J和C基因。HTS数据可作为指南，手动选择表达的V，D，J和C基因。雪貂的基因座似乎与狗最相似。像其他哺乳动物TRB基因座一样，雪貂TRB位点包含位于两个DJC基因簇上游的可变基因文库，其后是转录方向反向的（在雪貂无功能的）V基因。