Abstract

Different treatments were conducted over Ti6Al4V samples in order to produce a surface modification to increase cell attachment and proliferation. The surface treatments evaluated in this study were as follows: etching with sulfuric acid/hydrochloric acid, oxidizing with hydrogen peroxide and low-pressure oxygen plasma treatment. In contrast to other works found in the literature, this research conducts a comparison between different chemical and physical treatments in terms of different assays for surface characterization: X-ray diffraction, scanning electron microscope (SEM), energy-dispersive X-ray spectroscopy, water contact angle, release of vanadium ions and cell viability tests (MTT) of human osteoblasts (hFOB 1.19). Cell morphology over the different substrates was also studied by SEM observation. It was found that plasma and peroxide treatments increase the O/Ti ratio at the titanium surface and provide an increase in cell affinity. On the other hand, acid etching provides a superhydrophilic surface which is not able to improve the cell attachment of human osteoblasts.

Graphical abstract

中文翻译：

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低压氧等离子体与化学处理Ti6Al4V表面改性的比较

摘要

对Ti6Al4V样品进行了不同的处理，以产生表面修饰以增加细胞附着和增殖。本研究中评估的表面处理如下：用硫酸/盐酸蚀刻，用过氧化氢氧化和低压氧等离子体处理。与文献中发现的其他工作相比，本研究根据表面表征的不同分析方法对不同的化学和物理处理方法进行了比较：X射线衍射，扫描电子显微镜（SEM），能量色散X射线光谱，水接触角，钒离子释放和人类成骨细胞的细胞生存力测试（MTT）（hFOB 1.19）。还通过SEM观察研究了在不同底物上的细胞形态。发现等离子体和过氧化物处理增加了钛表面上的O / Ti比并提供了细胞亲和力的增加。另一方面，酸蚀刻提供了超亲水表面，该表面不能改善人成骨细胞的细胞附着。

图形概要