Abstract

Lantana camara, a noxious weed flourishing worldwide is one of the world’s 10 worst weed causing biological pollution. In this study, we amplified and sequenced stress responsive boiling stable protein encoding genes from leaves of L. camara. We also describe in-silico characterization and molecular modeling of LCDHY hydrophilic protein through homology search, secondary structure prediction, active site prediction and 3D structure analysis. Clustal W analysis showed the presence of conserved amino acid sequence. 3D structure was predicted using Swiss-model server and homology modeling. Structure was refined by energy minimization and stereo-chemical qualities validated with PROCHECK, VADAR, QMEAN servers. Active sites were predicted form target protein structure. NEST analysis revealed structural motifs in functionally important regions of protein structure. Based on gene ontology (GO) terms in ProFunc server, putative functions of modeled LCDHY protein were predicted.

中文翻译：

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外来入侵植物马tana丹（Lantana camara）的假定沸腾稳定蛋白编码基因的PCR扩增和硅内分析

摘要

马鞭草（Lantana camara）是一种在世界范围内蓬勃发展的有害杂草，是世界上造成生物污染最严重的10种杂草之一。在这项研究中，我们从L. camara的叶片中扩增并测序了胁迫响应沸腾稳定蛋白编码基因。我们还描述了硅通过同源搜索，二级结构预测，活性位点预测和3D结构分析对LCDHY亲水蛋白进行表征和分子建模。Clustal W分析表明存在保守的氨基酸序列。使用Swiss-model服务器和同源性建模来预测3D结构。通过最小化能量来优化结构，并通过PROCHECK，VADAR，QMEAN服务器验证立体化学质量。从靶蛋白结构预测活性位点。NEST分析显示蛋白质结构的功能重要区域中的结构基序。基于ProFunc服务器中的基因本体（GO）术语，预测了建模的LCDHY蛋白的推定功能。