In the modern world, healthy habits and physical and mental fitness are more important than ever. A growing number of people participate in sports to improve their overall health. However, the conditions in which people exercise are seldom examined. It is obvious that the air in buildings, including sports facilities, can be contaminated with pathogenic microorganisms, causing infections and allergies. Our study was aimed at assessing microbial air quality inside several sports facilities (fitness room, martial arts room, swimming pool, sports hall, gym) and at a sports field. Another objective was to evaluate the antibiotic resistance of isolated Staphylococcus strains. Air samples were collected with MAS-100 sampler, using selective substrates. Antibiotic resistance of mannitol-positive staphylococci was assessed using a disk diffusion method in accordance with EUCAST recommendations. The results indicated large fluctuations in average concentrations of heterotrophic bacteria, ranging from 38 CFU m−3 (swimming pool) to 1036 CFU m−3 (sports hall). Generally, bacteria were more abundant inside the buildings, while fungi in the sports field (658 CFU m−3 on average). In all facilities, airborne fungal communities were dominated by the genus Cladosporium, followed by Penicillium, Fusarium and Acremonium. Alternaria and Aureobasidium constituted only a small percentage of isolated molds. We recorded only low concentrations of mannitol-positive staphylococci (on average ranging from 1 CFU m−3 at the swimming pool and sports field to 9 CFU m−3 in the martial arts room). Of all isolated Staphylococcus strains, 73% were resistant to benzopenicillin, while more than 90% were sensitive to gentamycin, levofloxacin and rifampicin.

中文翻译：

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体育设施内生物气溶胶的微生物多样性和分离的葡萄球菌的抗生素耐药性。

在现代世界，健康的习惯和身心健康比以往任何时候都更加重要。越来越多的人参加体育运动以改善整体健康状况。然而，人们很少研究锻炼的条件。很明显，包括体育设施在内的建筑物内的空气都可能被病原微生物污染，引起感染和过敏。我们的研究旨在评估多个体育设施（健身房、武术室、游泳池、体育馆、健身房）和运动场内的微生物空气质量。另一个目的是评估分离的葡萄球菌菌株的抗生素抗性。使用 MAS-100 采样器收集空气样品，使用选择性底物。根据 EUCAST 建议，使用圆盘扩散法评估甘露醇阳性葡萄球菌的抗生素耐药性。结果表明异养细菌的平均浓度波动很大，范围从 38 CFU m-3（游泳池）到 1036 CFU m-3（运动场）。一般来说，建筑物内细菌较多，运动场内真菌较多（平均658 CFU m-3）。在所有设施中，空气传播的真菌群落以枝孢菌属为主，其次是青霉菌、镰刀菌和枝顶孢菌。Alternaria 和 Aureobasidium 仅占孤立霉菌的一小部分。我们只记录了低浓度的甘露醇阳性葡萄球菌（平均从游泳池和运动场的 1 CFU m-3 到武术室的 9 CFU m-3）。