Triplophysa siluroides is an endemic species to the upper reaches of the Yellow River. Due to over fishing and habitat destruction, the natural population of T. siluroides has decreased dramatically in recent years. Insufficient molecular markers have limited the effective conservation and management of this species. In the present study, we reported the isolation and characterization of 50 SNP markers in T. siluroides. The minor allele frequency ranged from 0.083 to 0.483, and the observed and expected heterozygosities ranged from 0.133 to 0.667 and 0.155 to 0.508, respectively. Polymorphic information content ranged from 0.141 to 0.399. Among these SNPs, only three loci showed significant departures from the Hardy–Weinberg equilibrium (P < 0.01). The novel polymorphic SNPs will be helpful for the future study on genetic management and population conservation for this species.

Triplophysa siluroides是黄河上游的特有物种。由于过度捕鱼和栖息地遭到破坏，近年来，T。siluroides的自然种群急剧减少。分子标记物不足限制了该物种的有效保存和管理。在本研究中，我们报道了T. siluroides中50个SNP标记的分离和鉴定。次要等位基因频率范围为0.083至0.483，观察到的和预期的杂合度分别为0.133至0.667和0.155至0.508。多态信息含量范围为0.141至0.399。在这些SNP中，只有三个基因座显示出与Hardy-Weinberg平衡显着偏离（P <0.01）。新颖的多态性SNP将有助于该物种的遗传管理和种群保护的未来研究。