The purpose of this study was to determine the anti-inflammatory and in vitro bone formation effects of Garcinia mangostana L. (mangosteen) and propolis extracts. Immortalized human gingival fibroblasts (hTERT-hNOF) cells were treated with Porphyromonas gingivalis KCOM 2804 lipopolysaccharide followed by treatment with mangosteen and propolis extract alone or in combination. Expression levels of inflammatory cytokines were evaluated by enzyme-linked immunosorbent assay. Effect of mangosteen and/or propolis extracts on mineralization of MG-63 cells was evaluated by alkaline phosphatase activity and alizarin red S staining. Group mangosteen extract complex 1:34 (1 µg/ml mangosteen extract and 34 µg/ml propolis extract) significantly reduced expression levels of IL-6, IL-8, and PGE2. It had higher than other groups in vitro bone formation effect on MG63 cells. These results suggest that mangosteen and propolis extract complex could be used in the prevention and treatment of periodontal disease.

中文翻译：

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藤黄和蜂胶提取物的抗炎和体外骨骼形成作用。

这项研究的目的是确定藤黄（mangostana L。）（山竹）和蜂胶提取物的抗炎和体外骨形成作用。用牙龈卟啉单胞菌KCOM 2804脂多糖处理永生化的人牙龈成纤维细胞（hTERT-hNOF），然后单独或组合用山竹和蜂胶提取物处理。通过酶联免疫吸附测定法评估炎性细胞因子的表达水平。通过碱性磷酸酶活性和茜素红S染色评价山竹和/或蜂胶提取物对MG-63细胞矿化的影响。组山竹提取物复合物1:34（1 µg / ml山竹提取物和34 µg / ml蜂胶提取物）显着降低IL-6，IL-8和PGE2的表达水平。它对MG63细胞的体外成骨作用高于其他组。这些结果表明，山竹和蜂胶提取物复合物可用于牙周疾病的预防和治疗。