Polyhydroxyalkanoates (PHAs) present an eco-friendly alternative for conventional plastics. Industrial wastewater from the food industry is a copious source of organic carbon that can be recovered in the form of PHA. However, the wastewater composition varies considerably among the different industries demanding for an industry-specific investigation of the PHA production process. Wastewater from the yeast industry, besides its high concentration in organic carbon, also contains a high ammonium concentration which might decrease the PHA production. Thus, this study aims to investigate PHA production using yeast industry wastewater via an enriched mixed microbial culture (MMC). A less explored cultivation strategy, i.e., continuous cultivation for PHA production was evaluated and compared to the widely used batch cultivation. PHA accumulating MMC was enriched using a sequencing batch reactor (SBR) operated under aerobic dynamic feeding. An MMC dominated by the Thauera species was successfully enriched in the SBR. Experimental results showed that ammonium is indeed required for PHA accumulation and the complete absence of ammonium negatively affects the accumulation process. Using wastewater, batch and continuous feeding strategies, respectively, yielded the PHA accumulation of 72 % and 65 % per dry cell weight. Despite the slightly lower PHA accumulation with continuous cultivation, four times more biomass growth was produced. Consequently, higher theoretical PHA production (270 t/year) can be expected using continuous cultivation in half of the reactor volume (45 m³). Therefore, this study asserts the viability of continuous cultivation as a feasible investigatory tool and PHA production strategy.

聚羟基链烷酸酯（PHA）为传统塑料提供了一种环保的替代品。食品工业废水是有机碳的丰富来源，可以PHA的形式回收。但是，不同行业之间的废水成分差异很大，需要对PHA生产过程进行行业特定的调查。酵母行业的废水，除了其高浓度的有机碳外，还含有高浓度的铵，这可能会降低PHA的产量。因此，本研究旨在研究通过酵母菌废水通过富集的混合微生物培养（MMC）生产PHA。评价较少探索的栽培策略，即用于PHA生产的连续栽培，并将其与广泛使用的分批栽培进行比较。使用在有氧动态进料下运行的测序间歇反应器（SBR）富集了PHA积累的MMC。由MMC主导的MMCThauera物种在SBR中成功富集。实验结果表明，PHA的积累确实需要铵盐，而完全不存在铵盐会对积累过程产生负面影响。使用废水时，分批和连续进料策略分别产生每干​​细胞重量72％和65％的PHA积累。尽管连续培养的PHA积累量略低，但产生的生物量却增长了四倍。因此，通过在反应器体积的一半（45 m ³）中进行连续培养，可以预期获得更高的理论PHA产量（270吨/年）。因此，本研究断言连续栽培作为可行的调查工具和PHA生产策略的可行性。