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Characterization of the core microbiome in tobacco leaves during aging.
MicrobiologyOpen ( IF 3.4 ) Pub Date : 2020-01-01 , DOI: 10.1002/mbo3.984
Jiaxi Zhou 1 , Lifei Yu 1, 2 , Jian Zhang 1 , Xiaomin Zhang 3 , Yuan Xue 4 , Jing Liu 5 , Xiao Zou 1
Affiliation  

Microbiome plays an important role during the tobacco aging process which was an indispensable link in the production and processing of cigarettes. However, the structure and functions of microbiome have not been clarified during the tobacco aging process. In this study, 16S rDNA and ITS amplicon sequencing techniques were used to analyze the core microbiome of 15 tobacco samples from five different aging stages. The whole bacterial microbiome was classified into 29 microbial phyla and 132 orders. Enterobacteriales (63%), Pseudomonadales (16%), Sphingomonadales (8%), Xanthomonadales (4%), Burkholderiales (4%), Rhizobiales (3%), and Bacillales (2%) comprised the core bacterial microbiome. The whole fungal microbiome was classified into five microbial phyla and 52 orders. Incertae_sedis_Eurotiomycetes (27%), Wallemiales (25%), Sporidiobolales (17%), Capnodiales (5%), Eurotiales (2%), an unclassified Ascomycota (12%), and an unidentified Eurotiomycetes (4%) comprised the core fungal microbiome. FAPROTAX function prediction suggested that the core microbiome has a substantial potential for the carbon cycle, nitrate metabolism, aromatic compound degradation, chitinolysis, cellulolysis, and xylanolysis, but simultaneously, the core microbiome is also a source of human pathogens. The dynamics of the bacterial community were primarily determined by the total nitrogen in tobacco leaves during the aging process, while those of the fungal microbiome were primarily determined by total organic carbon. This study indicated that the core microbiome activities may play an important role in regulating the loss of carbon organic compounds and enhancing the secondary metabolites during tobacco leaves aging process.

中文翻译:

烟叶老化过程中核心微生物组的表征。

微生物组在烟草老化过程中起着重要作用,而烟草老化过程是香烟生产和加工中必不可少的环节。但是,在烟草老化过程中,微生物组的结构和功能尚未阐明。在这项研究中,使用16S rDNA和ITS扩增子测序技术来分析来自五个不同老化阶段的15个烟草样品的核心微生物组。整个细菌微生物组分为29个微生物门和132个目。肠杆菌(63%),假单胞菌(16%),鞘氨醇(8%),黄单胞菌(4%),伯克霍尔德莱斯(4%),根瘤菌(3%)和芽孢杆菌(2%)构成了核心细菌微生物组。整个真菌微生物组分为五个微生物门和52个阶。Incertae_sedis_ Eurotiomycetes(27%),Wallemiales(25%),Sporidiobolales(17%),Capnodiales(5%),Eurotiales(2%),未分类的子囊菌(12%)和身份不明的Eurotiomycetes(4%)构成核心真菌微生物组。FAPROTAX功能预测表明,核心微生物组在碳循环,硝酸盐代谢,芳香族化合物降解,几丁质分解,纤维素分解和木聚糖分解方面具有巨大潜力,但同时,核心微生物组也是人类病原体的来源。细菌群落的动态主要取决于烟叶在衰老过程中的总氮,而真菌微生物组的动态主要取决于总有机碳。这项研究表明,核心的微生物组活性可能在调节烟叶老化过程中调节碳有机化合物的流失和增强次生代谢产物中起重要作用。
更新日期:2020-01-01
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