Fully automated system for sample preparation in molecular diagnostics was developed. The small cartridge with multiple chambers and a rotating valve was designed for automated processes such as extraction, separation and purifications during the DNA sample preparation. The automated processes such as injection, mixing, capturing and valve on/off motions were controlled by an affordable, motorized control system. We demonstrated the automated DNA extraction of pathogenic bacteria using this system. The deviations in the purity and real-time polymerase chain reaction (PCR) detection of the automated DNA extraction samples were clearly decreased. The device provided rapid, reliable, and reproducible sample preparation in comparison with manual DNA extraction. This automated platform can be utilized in emergent point-of-care testing of infectious disease and foodborne bacteria.

中文翻译：

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即时点（POC）分子诊断中快速，可重现样品制备的自动化平台

开发了用于分子诊断中样品制备的全自动系统。具有多个腔室和一个旋转阀的小盒设计用于DNA样品制备过程中的自动化过程，例如提取，分离和纯化。自动化过程（例如注射，混合，捕获和阀门开/关运动）由负担得起的电动控制系统控制。我们展示了使用该系统自动提取病原细菌的DNA。自动DNA提取样品的纯度和实时聚合酶链反应（PCR）检测的偏差明显减少。与手动DNA提取相比，该设备提供了快速，可靠且可重现的样品制备方法。