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An assessment of adaptive and antagonistic properties of Trichoderma sp. strains in vegetable waste composts
Archives of Environmental Protection ( IF 1.5 ) Pub Date : 2017-12-01 , DOI: 10.1515/aep-2017-0039
Agnieszka Wolna-Maruwka 1 , Tomasz Piechota 1 , Alicja Niewiadomska 1 , Jacek Dach 1 , Magdalena Szczech 1 , Małgorzata Jędryczka 1 , Agnieszka A. Pilarska 2
Affiliation  

Abstract The experiment consisted in monitoring the count of moulds and three selected Trichoderma sp. isolates (T1 - Trichoderma atroviride, T2 - Trichoderma harzianum, T3 - Trichoderma harzianum) in vegetable (onion and tomato) waste composted with additives (straw, pig manure). Additionally, the aim of the study was to determine the type of interaction occurring between autochthonous fungi isolated from composts after the end of the thermophilic phase and Trichoderma sp. strains applied in the experiment. Number of microorganisms was determined by the plate method, next the identification was confirmed. The rating scale developed by Mańka was used to determine the type of interactions occurring between microorganisms. The greatest count of moulds in onion waste composts was noted in the object which had simultaneously been inoculated with two strains T1 - T. atroviride and T3 - T. harzianum. The greatest count of moulds was noted in the tomato waste composts inoculated with T2 - T. harzianum strain. Microscope identification revealed that Penicillum sp., Rhizopus sp., Alternaria sp. and Mucor sp. strains were predominant in onion waste composts. In tomato waste composts Penicillium was the predominant genus, followed by Rhizopus. The test of antagonism revealed the inhibitory effect of Trichoderma isolates on most autochthonous strains of moulds. Tomato waste composts proved to be better substrates for the growth and development of Trichoderma sp. isolates. The results of the study show that vegetable waste can be used in agriculture as carriers of antagonistic microorganisms.

中文翻译:

木霉属适应性和拮抗特性的评估。植物废料堆肥中的菌株

摘要 该实验包括监测霉菌和三种选定的木霉属的数量。在与添加剂(稻草、猪粪)堆肥的蔬菜(洋葱和番茄)废物中分离(T1 - 木霉 atroviride,T2 - 哈茨木霉,T3 - 哈茨木霉)。此外,该研究的目的是确定在高温阶段结束后从堆肥中分离的本土真菌与木霉属之间发生的相互作用类型。实验中应用的菌株。用平板法测定微生物数,确​​认鉴定。Mańka 开发的评分量表用于确定微生物之间发生的相互作用类型。在同时接种了两种菌株 T1 - T. atroviride 和 T3 - T. harzianum 的对象中,洋葱废物堆肥中的霉菌数量最多。在接种了 T2 - T. harzianum 菌株的番茄废物堆肥中发现了最多的霉菌。显微镜鉴定显示青霉属、根霉属、链格孢属。和毛霉菌 sp。菌株在洋葱废物堆肥中占主导地位。在番茄废物堆肥中,青霉属是主要属,其次是根霉属。拮抗试验表明木霉分离株对大多数本土霉菌菌株具有抑制作用。番茄废物堆肥被证明是木霉属植物生长和发育的更好底物。隔离。
更新日期:2017-12-01
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