Lipid accumulation is generally believed to be a partially growth-coupled biochemical process that results in differences in lipid content between different cells. To separate lipid-rich cells and increase the cellular biomass in bioreactors during the cultivation of the oleaginous yeasts, a gravity sedimentation system (GSS) is coupled to a bioreactor. The dilution rate (D) and the ratio of the outflow rate from the outlet of the GSS to the inflow rate into the bioreactor (B) were evaluated. The biomass in the bioreactor with GSS increased by 16.3% and 30.6% at D values of 0.05 h⁻¹ (B = 0.25) and 0.02 h⁻¹ (B = 0.5), respectively. Interestingly, cells containing 39.3% lipids were obtained from the outlet of the GSS (D = 0.02 h⁻¹, B = 0.5), and the lipid content increased by 7.8% compared to that of the bioreactor. The results indicated that use of a GSS is a very effective method for increasing the cell concentration and separation of lipid-rich cells.

中文翻译：

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重力沉降系统连续培养的红景天产生脂质

通常认为脂质积累是部分生长偶联的生化过程，其导致不同细胞之间脂质含量的差异。为了在油脂酵母培养过程中分离富含脂质的细胞并增加生物反应器中的细胞生物量，将重力沉降系统（GSS）与生物反应器耦合。评价稀释率（D）和从GSS出口流出的流量与流入生物反应器的流量（B）之比。在D值为0.05 h ^-1（B  = 0.25）和0.02 h ^-1（B时 ，GSS生物反应器中的生物量分别增加了16.3％和30.6％。= 0.5）。有趣的是，从GSS的出口获得了含有39.3％脂质的细胞（D  = 0.02 h ^-1，B  = 0.5），与生物反应器相比，脂质含量增加了7.8％。结果表明，GSS的使用是增加细胞浓度和富脂质细胞分离的非常有效的方法。