Geomicrobiology is an interdisciplinary field which studies the impact of microbes in a given geologic environment. Besides understanding the role of microbes in processes such as mineral degradation, organic matter mineralization, and biogeochemical cycles, the presence of microbes as part of the indigenous population of contaminated areas can be used as a tool for in situ treatment and removal of heavy metals from contaminated sites. In order to isolate fungal bioprospects capable of growing on Copper (Cu) contaminated areas, samples were collected from soils in Puerto Rico. After performing serial dilutions, the samples were spread on plates supplemented with Cu (CuSO4) at concentrations from 0 - 1M. Only one microorganism was able to grow on medium supplemented with 35 mM Cu2+, a filamentous fungus of the genus Aspergillus. Taxonomic classification suggests the fungus was Aspergillus versicolor. At 35 mM of Cu, atypical growth of the fungi was observed, including changes in pigmentation and colony size. Hyphal morphology also changed and the presence of asexual reproductive structure such as conodiophores was not observed. In addition, Scanning Electron Microscopy study revealed the presence of spherical filamentous structures of unknown composition. Previous reports of Cu resistant Aspergillus sp. have described species such as A. niger and A. fumigatus, but to our knowledge this is the first report of an A. versicolor capable of growing at the Cu concentrations as high as the ones described in this study.

中文翻译：

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曲霉属的波多黎各分离株的表征。高铜电阻

地球微生物学是一个跨学科领域，研究微生物在给定地质环境中的影响。除了了解微生物在矿物降解，有机物矿化和生物地球化学循环等过程中的作用外，微生物作为受污染地区土著人口的一部分还可以用作原位处理和去除重金属的工具受污染的地点。为了分离能够在铜（Cu）污染地区生长的真菌生物前景，从波多黎各的土壤中收集了样品。进行系列稀释后，将样品以0-1M的浓度铺在补充有Cu（CuSO4）的板上。只有一种微生物能够在补充有35 mM Cu2 +（曲霉属的丝状真菌）的培养基上生长。分类学分类表明该真菌是杂色曲霉。在35 mM的Cu下，观察到真菌的非典型生长，包括色素沉着和菌落大小的变化。菌丝形态也发生了变化，未观察到无性生殖结构的存在，例如生食孔。此外，扫描电子显微镜研究发现存在未知成分的球形丝状结构。铜抗性曲霉菌的先前报道。我们已经描述了黑曲霉和烟曲霉等物种，但是据我们所知，这是第一本报道的杂色曲霉，能够以与本研究中所描述的铜一样高的浓度生长。菌丝形态也发生了变化，未观察到无性生殖结构的存在，例如生食孔。此外，扫描电子显微镜研究发现存在未知成分的球形丝状结构。铜抗性曲霉菌的先前报道。我们已经描述了黑曲霉和烟曲霉等物种，但是据我们所知，这是第一本报道的杂色曲霉，能够以与本研究中所描述的铜一样高的浓度生长。菌丝形态也发生了变化，未观察到无性生殖结构的存在，例如生食孔。此外，扫描电子显微镜研究发现存在未知成分的球形丝状结构。铜抗性曲霉菌的先前报道。我们已经描述了黑曲霉和烟曲霉等物种，但是据我们所知，这是第一本报道的杂色曲霉，能够以与本研究中所描述的铜一样高的浓度生长。