Immunoassay has been widely used in the screening of mycotoxins, which may be hazardous to the operator or the environment. This study was to develop a green way to measure zearalenone (ZEN) with a monoclonal β-type anti-idiotype antibody (Ab2β) against ZEN in place of ZEN standard. Six monoclonal β-type anti-idiotype antibodies were prepared. The 50% inhibitory concentration (IC₅₀) value to ZEN of the six antibodies was between 34.45 ± 1.12–182.12 ± 15.40 nM. A green ELISA was then developed and validated. The quantitative conversion formula between ZEN and the monoclonal Ab2β against ZEN was y = 0.092x^0.722, R² = 0.990. The working range was 2.63–100.64 ng ml⁻¹. The recovery rate in spiked feed samples was from 82.15% to 102.79%, and the within-assay and between-assay coefficient variation (CV) level were less than 10.00%. A good correlation was obtained by high-performance liquid chromatography method (HPLC) to validate the developed method.

免疫测定已广泛用于真菌毒素的筛选，这可能对操作者或环境造成危害。这项研究旨在开发一种绿色的方法，用抗ZEN的单克隆β型抗独特型抗体（Ab2β）代替ZEN标准来测量玉米赤霉烯酮（ZEN）。制备了六种单克隆β型抗独特型抗体。六种抗体对ZEN的50％抑制浓度（IC ₅₀）值在34.45±1.12–182.12±15.40 nM之间。然后开发并验证了绿色ELISA。ZEN和针对ZEN的单克隆Ab2β之间的定量转化公式为y  ＝ 0.092 × ^0.722，R ²＝ 0.990。工作范围是2.63–100.64 ng ml ^-1。加标饲料样品的回收率从82.15％到102.79％，测定内和测定间系数变化（CV）水平小于10.00％。通过高效液相色谱法（HPLC）获得了良好的相关性，以验证所开发的方法。